Medaka Oct4 is Essential for Pluripotency in Blastula Formation and ES Cell Derivation

文献类型： 外文期刊

第一作者： Liu, Rong

作者： Liu, Rong;Li, Mingyou;Li, Zhendong;Hong, Ni;Xu, Hongyan;Hong, Yunhan;Li, Mingyou;Hong, Yunhan;Hong, Ni;Xu, Hongyan

作者机构：

关键词： ES cell;Germ cell;Oct4;Pou2;Pluripotency

期刊名称：STEM CELL REVIEWS AND REPORTS （ 影响因子：5.739； 五年影响因子：5.171 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The origin and evolution of molecular mechanisms underlying cellular pluripotency is a fundamental question in stem cell biology. The transcription factor Oct4 or Pou5f1 identified in mouse features pluripotency expression and activity in the inner cell mass and embryonic stem (ES) cells. Pou2 identified in zebrafish is the non-mammalian homolog prototype of mouse Oct4. The genes oct4 and pou2 have reportedly evolved by pou5 gene duplication in the common ancestor of vertebrates. Unlike mouse oct4, however, zebrafish pou2 lacks pluripotency expression and activity. Whether the presence of pluripotency expression and activity is specific for mammalian Oct4 or common to the ancestor of vertebrate Oct4 and Pou2 proteins has remained to be determined. Here we report that Oloct4, the medaka oct4/pou2, is essential for early embryogenesis and pluripotency maintenance. Oloct4 exists as a single copy gene and is orthologous to pou2 by sequence and chromosome synteny. Oloct4 expression occurs in early embryos, germ stem cells and ES cells like mouse oct4 but also in the brain and tail bud like zebrafish pou2. Importantly, OlOct4 depletion caused blastula lethality or blockage. We show that Oloct4 depletion abolishes ES cell derivation from midblastula embryos. Thus, Oloct4 has pluripotency expression and is essential for early embryogenesis and pluripotency maintenance. Our results demonstrate the conservation of pluripotency expression and activity in vertebrate Oct4 and Pou2 proteins. The finding that Oloct4 combines the features of mouse oct4 and zebrafish pou2 in expression and function suggests that Oloct4 might represent the ancestral prototype of vertebrate oct4 and pou2 genes.

分类号： R31

  • 相关文献

[1]Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4. Li, Xiangchen,Guo, Yu,Yao, Yaxin,Ma, Yuehui,Liu, Changqing,Guan, Weijun,Guo, Yu,Liu, Changqing,Hua, Jinlian. 2016

[2]Culture of Chicken Embryonic Stem Cells in Buffalo Rat Liver 3A Cells (BRL-3A) Conditioned Medium. Zhang, Chuan-Sheng,Geng, Li-Ying,Du, Li-Xin. 2011

[3]Murine pluripotent stem cells that escape differentiation inside teratomas maintain pluripotency. Pei, Yangli,Pei, Yangli,Yue, Liang,Zhang, Wei,Xiang, Jinzhu,Han, Jianyong,Ma, Zhu. 2018

[4]Ultrastructure of germ cells, Sertoli cells and mitochondria during spermatogenesis in mature testis of the Chinese Taihang black goats (Capra hircus). Shi, Liguang,Xun, Wenjuan,Zhou, Hanlin,Hou, Guanyu,Shi, Liguang,Yue, Wenbin,Zhang, Chunxiang,Ren, Youshe,Yang, Rujie. 2013

[5]Barriers for Deriving Transgene-Free Pig iPS Cells with Episomal Vectors. Du, Xuguang,Feng, Tao,Yu, Dawei,Zou, Huiying,Ma, Shuangyu,Hu, Xiaoxiang,Li, Ning,Wu, Sen,Wu, Yuanyuan,Feng, Chong,Pan, Dengke,Huang, Yongye,Ouyang, Hongsheng.

[6]Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish. Ye, Huan,Li, Chuang-Ju,Yue, Hua-Mei,Yang, Xiao-Ge,Wei, Qi-Wei,Ye, Huan,Yang, Xiao-Ge,Wei, Qi-Wei,Li, Chuang-Ju,Yue, Hua-Mei,Wei, Qi-Wei.

[7]Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo. Rao, Feng,Wang, Tiansu,Li, Mingyou,Li, Zhendong,Hong, Ni,Zhao, Haobin,Yan, Yan,Lu, Wenqing,Chen, Tiansheng,Wang, Weijia,Lim, Menghuat,Yuan, Yongming,Hong, Yunhan,Yan, Yan,Li, Changming,Zhao, Haobin,Liu, Ling,Zeng, Lingbing,Wei, Qiwei,Hong, Yunhan,Guan, Guijun.

作者其他论文 更多>>

微信小程序