Characterization of the Macropodus opercularis complete mitochondrial genome and family Channidae taxonomy using Illumina-based de novo transcriptome sequencing

文献类型: 外文期刊

第一作者: Mu, Xidong

作者: Mu, Xidong;Liu, Yi;Lai, Mingxin;Song, Hongmei;Wang, Xuejie;Hu, Yinchang;Luo, Jianren

作者机构:

关键词: Macropodus opercularis;Mitochondrial genome;Phylogeny;De novo transcriptome sequencing

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

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收录情况: SCI

摘要: In this study, the complete mitochondrial genome of Macropodus opercularis was sequenced using Illumina-based de novo transcriptome technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,496 bp in length and contained two ribosomal RNAs, 13 protein-coding genes, 22 transfer RNA genes, and the control region. The gene composition and order were similar to suborder Anabantoidei. Phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Neighbor-joining and Bayesian analysis) both highly supported the close relationship of M. opercularis to M. ocellatus, consistent with previous classifications based on morphological and molecular studies. Furthermore, family Channidae and Parachanna insignis were clustered in the same clade. Our results supported the inclusion of family Channidae in suborder Channoidei. The complete mitochondrial genome of M. opercularis will provide genetic markers for better understanding species identification, population genetics and phylogeographics of freshwater fishes. (C) 2015 Elsevier B.V. All rights reserved.

分类号: R394

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