Proteomic characterization and comparison of mammalian milk fat globule proteomes by iTRAQ analysis

文献类型： 外文期刊

第一作者： Yang, Yongxin

作者： Yang, Yongxin;Zheng, Nan;Zhao, Xiaowei;Zhang, Yangdong;Han, Rongwei;Ma, Lu;Zhao, Shengguo;Li, Songli;Guo, Tongjun;Wang, Jiaqi;Yang, Yongxin;Zhao, Xiaowei;Zheng, Nan;Zhang, Yangdong;Zhao, Shengguo;Li, Songli;Wang, Jiaqi;Han, Rongwei

作者机构：

关键词： Milk fat globule membrane proteome;Quantitative proteomics;Mammal species;Clustering

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Milk fat globule membrane (MFGM) proteins are known to be involved in many biological functions; however, their components and inter-species complexity have not yet been completely elucidated. We investigated the protein composition of the MFGM-enriched fraction from Holstein, Jersey, yak, buffalo, goat, camel, horse, and human. Extracted proteins from the MFGM-enriched fractions were identified and quantified by an iTRAQ proteomic approach. We identified 520 protein species categorized as biological processes, cellular components and molecular function according to their annotation. Cellular process, localization, transport, signal transduction, and response to stimulus were the most common biological processes; binding and catalytic activities were the most prevalent molecular functions. Pathway analysis revealed several pathways, including glycolysis/ gluconeogenesis, peroxisome proliferator-activated receptor signaling, and fatty acid biosynthesis. Quantified MFGM-enriched proteins were subjected to discriminative proteomic profiling by principal component analysis and a hierarchical clustering method, and then organized into four major clusters: (1) Holstein, Jersey, and yak milk; (2) buffalo and goat milk; (3) Holstein, Jersey, buffalo, yak, and goat milk; and (4) camel, horse, and human milk. These novel quantitative data provide insight into the protein composition of the MFGM and their potential physiological functions, and highlight the significant differences in the MFGM fractions among mammalian species.

分类号： Q51

  • 相关文献

[1]Comparative proteomics of milk fat globule membrane in different species reveals variations in lactation and nutrition. Lu, Jing,Zhang, Weiqing,Liu, Lu,Pang, Xiaoyang,Zhang, Shuwen,Lv, Jiaping,Wang, Xinyu,Wang, Xinyu.

[2]Clustering and cellular distribution characteristics of virus particles of Tomato spotted wilt virus and Tomato zonate spot virus in different plant hosts. Zhang, Zhongkai,Wang, Xifeng,Zhang, Zhongkai,Zheng, Kuanyu,Dong, Jiahong,Fang, Qi,Hong, Jian. 2016

[3]Research on Spectra Recognition Method for Cabbages and Weeds Based on PCA and SIMCA. Zu Qin,Deng Wei,Wang Xiu,Zhao Chun-jiang,Zu Qin,Deng Wei,Wang Xiu,Zhao Chun-jiang,Zu Qin. 2013

[4]Pixel-Line Based Clustering for the 3D Point Cloud Generated by Kinect Depth Map. Qiu, Quan,Zheng, Wengang. 2013

[5]Proteomic analysis of cow, yak, buffalo, goat and camel milk whey proteins: Quantitative differential expression patterns. Yang, Yongxin,Bu, Dengpan,Zhao, Xiaowei,Sun, Peng,Wang, Jiaqi,Zhou, Lingyun,Yang, Yongxin,Zhao, Xiaowei.

[6]Quantitative proteomics by amino acid labeling in foot-and-mouth disease virus (FMDV)-infected cells. Ye, Yu,Xin, Chaoan,Liao, Ming,Fan, Huiying,Ye, Yu,Luo, Yongwen,Xin, Chaoan,Liao, Ming,Fan, Huiying,Ye, Yu,Xin, Chaoan,Liao, Ming,Fan, Huiying,Tong, Tiezhu,Yan, Guangrong,Yan, Guangrong,Liu, Xiangtao.

[7]Comparative analysis of primary hepatocellular carcinoma with single and multiple lesions by iTRAQ-based quantitative proteomics. Xing, Xiaohua,Huang, Yao,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Jingfeng,Xing, Xiaohua,Wang, Sen,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Minjie,Liu, Xiaolong,Liu, Jingfeng,Xing, Xiaohua,Wang, Sen,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Minjie,Liu, Xiaolong,Liu, Jingfeng,Han, Xiao.

[8]Proteome Differences between Hepatitis B Virus Genotype-B- and Genotype-C-Induced Hepatocellular Carcinoma Revealed by iTRAQ-Based Quantitative Proteomics. Wei, Dahai,Zeng, Yongyi,Xing, Xiaohua,Liu, Hongzhi,Lin, Minjie,Liu, Xiaolong,Liu, Jingfeng,Wei, Dahai,Zeng, Yongyi,Xing, Xiaohua,Liu, Hongzhi,Lin, Minjie,Liu, Xiaolong,Liu, Jingfeng,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao,Liu, Xiaolong,Liu, Jingfeng.

[9]Reveal the molecular signatures of hepatocellular carcinoma with different sizes by iTRAQ based quantitative proteomics. Wang, Yingchao,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Xing, Xiaohua,Xia, Jiangbao,Lin, Minjie,Liao, Naishun,Liu, Xiaolong,Liu, Jingfeng,Wang, Yingchao,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Xing, Xiaohua,Xia, Jiangbao,Lin, Minjie,Liao, Naishun,Liu, Xiaolong,Liu, Jingfeng,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao.

[10]Differential proteomic analysis of rice seedlings reveals the advantage of dry-raising nursery practices. Zhang, Zhixing,Huang, Fenglian,Chen, Hongfei,Lin, Wenxiong,Zhang, Zhixing,Huang, Fenglian,Chen, Hongfei,Lin, Wenxiong,Shao, CaiHong. 2018

[11]Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann. Ji, Ting,Liu, Zhenguo,Shen, Jie,Shen, Fang,Chen, Guohong,Liang, Qin,Wu, Liming,Corona, Miguel. 2014

[12]Proteomic analysis of sheep primary testicular cells infected with bluetongue virus. Du, Junzheng,Xing, Shanshan,Tian, Zhancheng,Gao, Shandian,Xie, Junren,Chang, Huiyun,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[13]Comparative Proteomic Analysis of Wild-Type and SAP Domain Mutant Foot-and-Mouth Disease Virus-Infected Porcine Cells Identifies the Ubiquitin-Activating Enzyme UBE1 Required for Virus Replication. Zhu, Zixiang,Yang, Fan,Zhang, Keshan,Cao, Weijun,Jin, Ye,Wang, Guoqing,Mao, Ruoqing,Li, Dan,Guo, Jianhong,Liu, Xiangtao,Zheng, Haixue.

[14]Quantitative proteomics of Sesuvium portulacastrum leaves revealed that ion transportation by V-ATPase and sugar accumulation in chloroplast played crucial roles in halophyte salt tolerance. Yi, Xiaoping,Sun, Yong,Yang, Qian,Guo, Anping,Chang, Lili,Wang, Dan,Tong, Zheng,Jin, Xiang,Wang, Limin,Wang, Xuchu,Yi, Xiaoping,Guo, Anping,Chang, Lili,Wang, Xuchu,Yu, Jianlan,Jin, Wenhai.

[15]Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique. Sun, Dongbo,Shi, Hongyan,Chen, Jianfei,Shi, Da,Zhang, Xin,Feng, Li,Sun, Dongbo,Guo, Donghua,Zhu, Qinghe.

[16]Proteomic analysis of conidia germination in Fusarium oxysporum f. sp cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Deng, Gui-Ming,Yang, Qiao-Song,He, Wei-Di,Li, Chun-Yu,Yang, Jing,Zuo, Cun-Wu,Gao, Jie,Sheng, Ou,Yi, Gan-Jun,Deng, Gui-Ming,Yang, Qiao-Song,He, Wei-Di,Li, Chun-Yu,Yang, Jing,Zuo, Cun-Wu,Gao, Jie,Sheng, Ou,Yi, Gan-Jun,Deng, Gui-Ming,Yang, Jing,Zuo, Cun-Wu,Gao, Jie,Lu, Shao-Yun,Yi, Gan-Jun,He, Wei-Di,Zhang, Sheng.

[17]iTRAQ, analysis of the tobacco leaf proteome reveals that RNA-directed DNA methylation (RdDM) has important roles in defense against geminivirus-betasatellite infection. Wang, Zhan Qi,Xiao, Ruyuan,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Zhou, Xueping.

作者其他论文 更多>>

微信小程序