THERMOLYSIN DAMAGES ANIMAL LIFE THROUGH DEGRADATION OF PLASMA PROTEINS ENHANCED BY RAPID CLEAVAGE OF SERPINS AND ACTIVATION OF PROTEASES

文献类型： 外文期刊

第一作者： Kong, Lulu

作者： Kong, Lulu;Liu, Chaoliang;Kong, Lulu;Kong, Lulu;Lu, Anrui;Guan, Jingmin;Yang, Bing;Ling, Erjun;Li, Muwang;Hillyer, Julian F.;Hillyer, Julian F.;Ramarao, Nalini;Soderhall, Kenneth

作者机构：

关键词： thermolysin;plasma;cleavage

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Thermolysin, a metallopeptidase secreted by pathogenic microbes, is concluded as an important virulence factor due to cleaving purified host proteins in vitro. Using the silkworm Bombyx mori as a model system, we found that thermolysin injection into larvae induces the destruction of the coagulation response and the activation of hemolymph melanization, which results in larval death. Thermolysin triggers the rapid degradation of insect and mammalian plasma proteins at a level that is considerably greater than expected in vitro and/or in vivo. To more specifically explore the mechanism, thermolysin-induced changes to key proteins belonging to the insect melanization pathway were assessed as a window for observing plasma protein cleavage. The application of thermolysin induced the rapid cleavage of the melanization negative regulator serpin-3, but did not directly activate the melanization rate-limiting enzyme prophenoloxidase (PPO) or the terminal serine proteases responsible for PPO activation. Terminal serine proteases of melanization are activated indirectly after thermolysin exposure. We hypothesize that thermolysin induces the rapid degradation of serpins and the activation of proteases directly or indirectly, boosting uncontrolled plasma protein degradation in insects and mammalians.

分类号： Q966

  • 相关文献

[1]Characterization of target mRNAs for Prunus persica microRNAs using an integrated strategy of PLM-RACE, PPM-RACE and qRT-PCR. Zhang, Yanping,Han, Jian,Pervaiz, Tariq,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan. 2014

[2]Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica. Zhang, Chunhua,Guo, Lei,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Li, Xiaoying. 2012

[3]Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris. Wang, Sanhong,Yao, Quanhong,Tao, Jianmin,Qiao, Yushan,Zhang, Zhen.

[4]Comparative blood biochemistry of Amur sturgeon, Acipenser schrenckii, and Chinese surgeon, Acipenser sinensis. Shi, Xiaotao,Li, Dapeng,Zhuang, Ping,Nie, Fen,Long, Liangqi. 2006

[5]Simultaneous Detection of 21 Kinds of Mycotoxins and Their Metabolites in Animal Plasma with Impurity Adsorption Purification Followed by Liquid Chromatography-Tandem Mass Spectrometry. Wang Rui-Guo,Su Xiao-Ou,Wang Pei-Long,Zhang Wei,Xue Yan,Li Yu. 2017

[6]Effect of supplemental beta-carotene in the periparturient diet on plasma vitamin A and beta-carotene concentrations and lymphocyte proliferation in Holstein cows. He, W. J.,Ren, L. P.,Meng, Q. X.,He, W. J.,Ren, L. P.,Meng, Q. X.,Zhang, Q. D.. 2007

[7]Comparative Proteomic Analysis of Plasma from Clinical Healthy Cows and Mastitic Cows. Yang Yong-xin,Zhao Xing-xu,Zhang Yong,Yang Yong-xin. 2009

[8]Plasma pharmacokinetics of melamine and a blend of melamine and cyanuric acid in rainbow trout (Oncorhynchus mykiss). Xue, Min,Qin, Yuchang,Wang, Jia,Wu, Xiufeng,Zheng, Yinhua,Wang, Qiuyan,Xue, Min,Qiu, Jing. 2011

[9]Application of a NMR-based untargeted quantitative metabonomic approach to screen for illicit salbutamol administration in cattle. Tang, Chaohua,Zhang, Kai,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin.

[10]Study on an antifouling and blood compatible poly(ethylene-vinyl acetate) material with fluorinated surface structure. Wen, Xiao-Wen,Pei, Su-Peng,Li, Hong,Ai, Fei,Chen, Huan,Zhang, Yong-Ming,Li, Ke-Yong,Wang, Quan.

[11]In-tube solid-phase microextraction based on hybrid silica monolith coupled to liquid chromatography-mass spectrometry for automated analysis of ten antidepressants in human urine and plasma. Zheng, Ming-Ming,Wang, Shao-Ting,Hu, Wei-Kang,Feng, Yu-Qi,Zheng, Ming-Ming.

[12]Non-thermal plasma assisted polymer surface modification and synthesis: A review. Cheng Yanling,Cheng Yanling,Chen, Paul,Deng, Shaobo,Ruan, Roger,Wang Yingkuan. 2014

[13]ITRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation. Ma Lu,Yan Su-mei,Ma Lu,Bu Deng-pan,Yang Yong-xing,Wang Jia-qi,Bu Deng-pan. 2015

[14]Zinc Oxide Nanoparticle Caused Plasma Metabolomic Perturbations Correlate with Hepatic Steatosis. Zhang, Weidong,Zhao, Yong,Li, Lan,Feng, Yanni,Min, Lingjiang,Ma, Dongxue,Yu, Shuai,Shen, Wei,Zhao, Yong,Zhang, Hongfu,Li, Fuli,Liu, Jing,Shi, Tianhong,Li, Fuwei. 2018

[15]Isobaric Tags for Relative and Absolute Quantification (iTRAQ)-Based Untargeted Quantitative Proteomic Approach To Identify Change of the Plasma Proteins by Salbutamol Abuse in Beef Cattle. Zhang, Kai,Tang, Chaohua,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin,Zhang, Kai,Tang, Chaohua,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin. 2018

[16]Comparative proteomics analysis provide novel insight into laminitis in Chinese Holstein cows. Dong, Shu-Wei,Zhang, Shi-Dong,Wang, Dong-Sheng,Wang, Hui,Shang, Xiao-Fei,Yan, Zuo-Ting,Yang, Zhi-Qiang,Dong, Shu-Wei,Yan, Ping. 2015

[17]Effects of duodenal infusion of free alpha-linolenic acid on the plasma and milk proteome of lactating dairy cows. Yang, Y. X.,Wang, J. Q.,Yuan, T. J.,Bu, D. P.,Yang, J. H.,Sun, P.,Zhou, L. Y.,Yang, Y. X.. 2013

[18]Study on Influences of Enzymolysis and Organic Solvent Extraction on Determination of 2 Kinds of beta(2)-Agonist Residues in Sheep Plasma and Urine. Li Yang,Su Xiao-Ou,Zhang Wei,Fan Xia,Wang Pei-Long,Wang Rui-Guo,Wang Xiao. 2014

[19]Surface Modification of Natural Rubber Latex Film by N-Vinypyrrolidone Graft Copolymerization. Sun, Qin,Yu, Heping,Zeng, Zongqiang,Lu, Guang,Luo, Yongyue. 2012

[20]Optimal starvation time before blood sampling to get baseline data on several blood biochemical parameters in Amur sturgeon, Acipenser schrenckii. Shi, X.,Zhuang, P.,Zhang, L.,Xu, B.,Feng, G.,Huang, X.,Shi, X.,Zhuang, P.,Chen, L.,Shi, X.. 2010

作者其他论文 更多>>

微信小程序