Highly selective colorimetric bacteria sensing based on protein-capped nanoparticles

文献类型： 外文期刊

第一作者： Qiu, Suyan

作者： Qiu, Suyan;Zhou, Yaomin;Wang, Donggen;Yuan, Lijuan;Wei, Yihua;Dai, Tingcan;Luo, Linguang;Qiu, Suyan;Lin, Zhenyu;Chen, Guonan

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期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）

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收录情况： SCI

摘要： A rapid and cost-effective colorimetric sensor has been developed for the detection of bacteria (Bacillus subtilis was selected as an example). The sensor was designed to rely on lysozyme-capped AuNPs with the advantages of effective amplification and high specificity. In the sensing system, lysozyme was able to bind strongly to Bacillus subtilis, which effectively induced a color change of the solution from light purple to purplish red. The lowest concentration of Bacillus subtilis detectable by the naked eye was 4.5 x 10(3) colony-forming units (CFU) mL(-1). Similar results were discernable from UV-Vis absorption measurements. A good specificity was observed through a statistical analysis method using the SPSS software (version 17.0). This simple colorimetric sensor may therefore be a rapid and specific method for a bacterial detection assay in complex samples.

分类号： O65

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