Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene
文献类型: 外文期刊
第一作者: Chen, Jia-zeng
作者: Chen, Jia-zeng;Peng, Jin-mei;Bai, Yun;Wang, Qian;Liu, Yi-min;Zhang, Qiu-yue;Chang, Dan;Zhang, Wu-chao;Zhao, Hong-yuan;Ye, Chao;An, Tong-qing;Cai, Xue-hui;Tian, Zhi-jun;Tong, Guang-zhi
作者机构:
关键词: PRRSV;GP2;Genetic diversity
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) strains (Henan-A10 and A11) were isolated from the sera of aborting sows. Interestingly, both of the isolates could replicate in primary porcine alveolar macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on the complete genome was constructed and the results showed that Henan-A10 and A11 were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. However, genomic sequence analysis showed that Henan-A10 and A11 shared only 96.8-97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain from a HP-PRRSV) was used to construct a chimeric clone with the corresponding deletion in GP2. We found that the deletion did not affect viral growth in MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable. (C) 2015 Elsevier B.V. All rights reserved.
分类号: S8
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