Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene

文献类型: 外文期刊

第一作者: Chen, Jia-zeng

作者: Chen, Jia-zeng;Peng, Jin-mei;Bai, Yun;Wang, Qian;Liu, Yi-min;Zhang, Qiu-yue;Chang, Dan;Zhang, Wu-chao;Zhao, Hong-yuan;Ye, Chao;An, Tong-qing;Cai, Xue-hui;Tian, Zhi-jun;Tong, Guang-zhi

作者机构:

关键词: PRRSV;GP2;Genetic diversity

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) strains (Henan-A10 and A11) were isolated from the sera of aborting sows. Interestingly, both of the isolates could replicate in primary porcine alveolar macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on the complete genome was constructed and the results showed that Henan-A10 and A11 were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. However, genomic sequence analysis showed that Henan-A10 and A11 shared only 96.8-97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain from a HP-PRRSV) was used to construct a chimeric clone with the corresponding deletion in GP2. We found that the deletion did not affect viral growth in MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable. (C) 2015 Elsevier B.V. All rights reserved.

分类号: S8

  • 相关文献

[1]Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013. Leng, Chao-liang,Sun, Long,Zhang, Gui-hong,Tong, Guang-zhi,Leng, Chao-liang,Tian, Zhi-jun,Zhang, Wu-chao,Zhang, Hong-liang,Zhai, Hong-yue,An, Tong-qing,Peng, Jin-mei,Ye, Chao,Wang, Qian,Sun, Yan,Li, Lin,Zhao, Hong-yuan,Chang, Dan,Cai, Xue-hui,Tong, Guang-zhi,Tong, Guang-zhi.

[2]Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain. Jiang, Yi-feng,Xia, Tian-qi,Zhou, Yan-jun,Yu, Ling-xue,Yang, Shen,Huang, Qin-feng,Li, Li-wei,Gao, Fei,Qu, Ze-hui,Tong, Wu,Tong, Guang-zhi,Jiang, Yi-feng,Zhou, Yan-jun,Tong, Guang-zhi.

[3]Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Yan, Li-Ping,Xu, Ao-Tian,Wang, Ya-Xin,Zhang, Shan-Rui,Tong, Guang-Zhi,Tian, Zhi-Jun,Hao, Xiao-Fang,Peng, Jin-Mei,An, Tong-Qing,Wei, Tian-Chao,Cai, Xue-Hui,Feng, Li,Li, Xi,Zhang, Gui-Hong,Zhou, Lun-Jiang. 2009

[4]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[5]Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs. Du, Yijun,Song, Cheng,Yoo, Dongwan,Pei, Yanlong,Hodgins, Douglas C.,Wu, Jiaqiang,Song, Cheng,Welch, Siao-Kun W.,Calvert, Jay G.,Li, Gang. 2009

[6]Identification of immunodominant T-cell epitopes in membrane protein of highly pathogenic porcine reproductive and respiratory syndrome virus. Tong, Guang-zhi. 2011

[7]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[8]Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus. Zhang, Shanrui,Zhou, Yanjun,Jiang, Yifeng,Li, Guoxin,Yan, Liping,Yu, Hai,Tong, Guangzhi. 2011

[9]Interference of porcine reproductive and respiratory syndrome virus replication on MARC-145 cells using DNA-based short interfering RNAs. He, Yun-xia,Hua, Rong-hong,Zhou, Yan-jun,Qiu, Hua-ji,Tong, Guang-zhi. 2007

[10]Mapping codon usage of the translation initiation region in porcine reproductive and respiratory syndrome virus genome. Su, Jun-hong,Li, Ji-dong,Ma, Xu-sheng,Dou, Yong-xi,Luo, Xue-nong,Cai, Xue-peng,Ma, Xiao-xia,He, Ya-li,Li, Ji-dong. 2011

[11]Enhanced immunogenicity induced by an alphavirus replicon-based pseudotyped baculovirus vaccine against porcine reproductive and respiratory syndrome virus. Wu, Qunfeng,Xu, Fengqin,Fang, Liurong,Xu, Jinfang,Li, Bin,Jiang, Yunbo,Chen, Huanchun,Xiao, Shaobo,Li, Bin. 2013

[12]The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages. Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Shi, Jianli,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Liu, Yanyan,Zhu, Xiwang,Wu, Jiaqiang,Wang, Jinbao,Liu, Xing. 2017

[13]Stable expression of foreign gene in nonessential region of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus: Applications for marker vaccine design. Xu, Yan-Zhao,Zhou, Yan-Jun,Zhang, Shan-Rui,Jiang, Yi-Feng,Tong, Wu,Yu, Hai,Tong, Guang-Zhi.

[14]Influence of N-linked glycosylation of minor proteins of porcine reproductive and respiratory syndrome virus on infectious virus recovery and receptor interaction. Wei, Zuzhang,Tian, Debin,Sun, Lichang,Lin, Tao,Gao, Fei,Liu, Runxia,Tong, Guangzhi,Yuan, Shishan.

[15]Hemagglutinating virus of Japan envelope (HVJ-E) can enhance the immune responses of swine immunized with killed PRRSV vaccine. Dai, Zhihong,Zhang, Zhongqiu,Zhao, Deming,Dai, Zhihong,Wang, Zaishi,Zhang, Quan,Zhang, Zhongqiu,Guo, Pengju. 2011

[16]Production of porcine TNF alpha by ADAM17-mediated cleavage negatively regulates porcine reproductive and respiratory syndrome virus infection. Li, Ren,Guo, Longjun,Gu, Weihong,Luo, Xiaolei,Zhang, Jian,Xu, Yunfei,Tian, Zhijun,Feng, Li,Wang, Yue.

[17]Cloning and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus of the ZJ Strain. Hao, Baocheng,Niu, Dengyuan,Xing, Xiaoyong,Xiang, Haitao,Wen, Fengqin,Fu, Xiaoping,Liang, Jianping,Hu, Yonghao,Niu, Dengyuan,Liang, Jianping. 2014

[18]Production of antisera against porcine haptoglobin: Potential for distinguishing haptoglobin subunits. Yang, Yongqian,An, Tongqing,Liu, Fei,Yuan, Zhonghua,Peng, Jinmei,Wu, Yuquan,Meng, Zhenxiang,Tian, Zhijun,Yang, Yongqian,Wu, Jiang,Zhang, Deli.

[19]Nonstructural Protein 4 of Porcine Reproductive and Respiratory Syndrome Virus Modulates Cell Surface Swine Leukocyte Antigen Class I Expression by Downregulating beta 2-Microglobulin Transcription. Qi, Pengfei,Liu, Ke,Wei, Jianchao,Li, Yuming,Li, Beibei,Shao, Donghua,Wu, Zhuanchang,Shi, Yuanyuan,Tong, Guangzhi,Qiu, Yafeng,Ma, Zhiyong.

[20]Open reading frames 1a and 1b of the porcine reproductive and respiratory syndrome virus (PRRSV) collaboratively initiate viral minus-strand RNA synthesis. Tang, Yan-Dong,Fang, Qiong-Qiong,Liu, Ji-Ting,Wang, Tong-Yun,Wang, Yu,Tao, Ye,Liu, Yong-gang,Cai, Xue-Hui,Liu, Ji-Ting.

作者其他论文 更多>>