Isolation, characterization and expression analysis of the GDP dissociation inhibitor protein gene MiRab-GDI from Mangifera indica L

文献类型： 外文期刊

第一作者： Liu, Zhaoliang

作者： Liu, Zhaoliang;Luo, Cong;Li, Lishu;Doug, Long;Can, Vantoan;Wei, Pengxiao;He, Xinhua;He, Xinhua;Liu, Zhaoliang

作者机构：

关键词： MiRab-GDI;Mangifera indica;Fruit ripening;Abiotic stresses

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Rab guanosine nucleotide dissociation inhibitor (Rab-GDI) proteins are important Rab GTPase effectors, which control the cycling of small GTPases between membrane and cytosol byinteracting with the prenylated GDP bound small GTPases. However, little is known about the function of Rab-GDIs in fruit plants. In this study, we present the cloning and characterisation of a putative MiRab-GDI (GenBank accession no. KF768565) from Mangifera indica. The gene encoded a 444 amino acid protein with a molecular weight of 49.75 kDa and a theoretical isoelectric point of 5.48. The deduced amino acid sequence exhibited high homology with Nicotiana tabacum (92% similarity) and contained five structurally and functionally defined sequence conserved residues (SCRs). SOS-PAGE and Western blot analysis indicated that recombinant MiRab-GDI was successfully expressed in E. coli and the molecular weight of the solute protein was the same as the predicted value. Real-time RT-PCR analysis demonstrated that MiRab-GDI was ubiquitously expressed in various plant tissues and organs at different levels. The expression of MiRab-GDI was down-regulated in the early stages of fruit development and up-regulated during later stages of fruit ripening. In addition, it could be induced by various treatments such as cold, NaCl, drought, abscisic acid (ABA), salicylic acid (SA), and hydrogenperoxide (H2O2). These results provide insights into the role of the MiRab-GDI gene in fruit ripening and abiotic stresses in the mango plants. (C) 2015 Elsevier B.V. All rights reserved.

分类号： S6

  • 相关文献

[1]Development of a sensitive molecular detection assay for mango malformation disease caused by Fusarium mangiferae. Wu, Jingbo,Liu, Feng,Zhan, Rulin,Li, Guoping,Zhao, Yanlong,Chang Jinmei,He, Yanbiao.

[2]An improved method to extract DNA from mango Mangifera indica. Uddin, Mohammad S.,Sun, Wenli,Cheng, Qi,Uddin, Mohammad S.,He, Xinhua.

[3]First Report of Dieback of Mango Caused by Fusarium decemcellulare in China. Qi, Yan-Xiang,Pu, Jin-Ji,Zhang, Xin,Zhang, He,Lu, Ying,Yu, Qun-Fang,Zhang, Hui-Qiang,Xie, Yi-Xian,Qi, Yan-Xiang,Pu, Jin-Ji,Zhang, Xin,Zhang, He,Lu, Ying,Yu, Qun-Fang,Zhang, Hui-Qiang,Xie, Yi-Xian,Qi, Yan-Xiang,Pu, Jin-Ji,Zhang, Xin,Zhang, He,Lu, Ying,Yu, Qun-Fang,Zhang, Hui-Qiang,Xie, Yi-Xian. 2013

[4]Cryopreservation of mango (Mangifera indica L.) embryogenic cultures. Wu, YJ,Huang, XL,Xiao, JN,Li, XJ,Zhou, MD,Engelmann, F. 2003

[5]The laccase gene (LAC1) is essential for Colletotrichum gloeosporioides development and virulence on mango leaves and fruits. Wei, Yunxie,Liu, Yanan,Zhou, Fangxue,Zhang, Kaili,Liu, Xiaomei,Wei, Yunxie,Liu, Yanan,Zhou, Fangxue,Zhang, Kaili,Liu, Xiaomei,Pu, Jinji,Zhang, He. 2017

[6]Fusarium proliferatum Caused Mango Malformation Disease in panzhihua and Huaping Provinces of China. Lv, Y. C.,Pu, J. J.,Qia, Y. X.,Xie, Y. X.,Lu, Y.,Zhang, X.,Zhang, H.,Zhang, H. Q..

[7]Construction of Progeny Population with Resistance to Mango Anthracnose and Their Identification by SSRs. Yao, Q. S.,Ma, X. W.,Lei, X. T.,Zhan, R. L.,Wang, S. B.,Wu, H. X..

[8]Mango Malformation Disease in South China Caused by Fusarium proliferatum. Zhan, Ru-Lin,Yang, Shun-Jin,Liu, Feng,Zhao, Yan-Long,Chang, Jin-Mei,He, Yan-Biao,Ho, Hon-Hing.

[9]Transcriptional sequencing and analysis of major genes involved in the adventitious root formation of mango cotyledon segments. Li, Yun-He,Zhang, Hong-Na,Wu, Qing-Song,Li, Yun-He,Li, Yun-He,Muday, Gloria K..

[10]A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation. Guo, Jun-E,Hu, Zongli,Yu, Xiaohui,Li, Anzhou,Li, Fenfen,Wang, Yunshu,Chen, Guoping,Tian, Shibing. 2018

[11]Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening. Kuang, Jian-fei,Chen, Lei,Xie, Hui,Peng, Huan-huan,Xiao, Yun-yi,Li, Xue-ping,Chen, Wei-xin,Chen, Jian-ye,Lu, Wang-jin,He, Quan-guang. 2012

[12]Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening. Ye Shu-e,Li Fang,Li Xian-bi,Zhai Yun-lan,Hu Ming-yu,Wei Ting,Deng Sha-sha,Pei Yan,Luo Ming,Hong Qi-bin. 2015

[13]Identification of Candidate Anthocyanin-Related Genes by Transcriptomic Analysis of `Furongli' Plum (Prunus salicina Lindl.) during Fruit Ripening Using RNA-Seq. Fang, Zhi-Zhen,Zhou, Dan-Rong,Ye, Xin-Fu,Jiang, Cui-Cui,Pan, Shao-Lin. 2016

[14]Functional analysis of differentially expressed proteins in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits during ripening. Chen, Yi-Yong,Zhong, Can-Yu,Song, Xiao-Min,Huang, Chun-Mei,Huang, Rong-Hui,Chen, Wei,Zhang, Ze-Huang,Lin, Qi-Hua,Chen, Yi-Yong,Chen, Wei. 2016

[15]Identification of miRNAs involved in fruit ripening in Cavendish bananas by deep sequencing. Bi, Fangcheng,Meng, Xiangchun,Yi, Ganjun,Bi, Fangcheng,Meng, Xiangchun,Yi, Ganjun,Bi, Fangcheng,Meng, Xiangchun,Yi, Ganjun,Ma, Chao. 2015

[16]Molecular Cloning, Structural Characterization, and Ripening-Related Expression of Lipoxygenase Genes from Three Persimmon Cultivars Differing in Postharvest Ripening Rate. Hou, Yali,Ban, Qiuyan,Zhang, Ting,Rao, Jingping,Huber, Donald J.,Dong, Xiaoqing,Chang, Xiaoxiao,Zhang, Ting.

[17]Genome-wide identification and expression analysis of the lipoxygenase gene family during peach fruit ripening under different postharvest treatments. Guo, Shaolei,Song, Zhizhong,Ma, Ruijuan,Yang, Yong,Yu, Mingliang,Guo, Shaolei,Yang, Yong,Guo, Shaolei,Song, Zhizhong,Ma, Ruijuan,Yang, Yong,Yu, Mingliang.

[18]Difference in activity and gene expression of pectin-degrading enzymes during softening process in two cultivars of Chinese pear fruit. Wei, Jianmei,Wei, Jianmei,Cheng, Yudou,Guan, Junfeng,Qi, Xiudong,Cheng, Yudou,Guan, Junfeng.

[19]Reduction of postharvest anthracnose and enhancement of disease resistance in ripening mango fruit by nitric oxide treatment. Hu, Meijiao,Yang, Dongping,Li, Min,Gao, Zhaoyin,Zhang, Zhengke,Huber, Donald J.,Jiang, Yueming,Zhang, Zhengke.

[20]Identification of a Gene Encoding Glutamate Decarboxylase Involved in the Postharvest Fruit Ripening Process in Banana. Hu, Wei,Liu, Ju-Hua,Yang, Xiao-Ying,Zhang, Jian-Bin,Jia, Cai-Hong,Li, Mei-Ying,Xu, Bi-Yu,Jin, Zhi-Qiang,Jin, Zhi-Qiang.

作者其他论文 更多>>

微信小程序