Co-silence of the coatomer beta and v-ATPase A genes by siRNA feeding reduces larval survival rate and weight gain of cotton bollworm, Helicoverpa armigera

文献类型： 外文期刊

第一作者： Zeng, Fanrong

作者： Zeng, Fanrong;Zhang, Peizhuo;Liu, Changyan

作者机构：

关键词： RNAi;siRNA;Helicoverpa armigera

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

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收录情况： SCI

摘要： Coatomer and v-ATPase are two genes expressed in insect midgut epithelial cells and their knockdown is lethal to insect larvae. To investigate the RNAi response mediated by multiple siRNA duplexes, partial length cDNA of Helicoverpa armigera coatomer beta and v-ATPase A was cloned and siRNA feeding-based RNAi was performed. Simultaneous ingestion of siRNAs specific to the H. armigera coatomer beta and v-ATPase A led to co-silencing of the target genes and reduction in larval survival rate and weight gain. These results suggest that silencing two genes by feeding of multiple siRNAs is a good RNAi strategy. (C) 2014 Elsevier Inc. All rights reserved.

分类号： S4

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