An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody
文献类型: 外文期刊
第一作者: Fang, Qingkui
作者: Fang, Qingkui;Wang, Limin;Hua, Xiude;Wang, Yulong;Wang, Suyan;Cheng, Qi;Cai, Jia;Liu, Fengquan;Liu, Fengquan
作者机构:
关键词: Butocarboxim;Oxime moiety;Monoclonal antibody;Enzyme-linked chemiluminescent immunoassay
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN:
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收录情况: SCI
摘要: In this study, four different haptens around the oxime moiety of Butocarboxim were designed and synthesised. Two of the haptens were conjugated with bovine serum albumin (BSA) to serve as the immunogen and all the haptens were conjugated with ovalbumin (OVA) for the coating antigen. The anti-Butocarboxim monoclonal antibody (Mab) was selected based on eight immunogen/coating antigen combinations. The first enzyme-linked chemiluminescent immunoassay (ELCIA) for determining Butocarboxim in agricultural products was developed. Under the optimised conditions, the detection limit for the ELCIA was 20 ng.mL(-1) and the linear range was 27-2700 ng.mL(-1). Analyte recoveries for extracts of spiked agricultural (apple and greengrocery) products and tap water ranged from 97.18% to 107.00%. The developed immunoassay has great potential to be developed as a test kit offering a simple and cost-effective approach (such as lateral flow test strip) for screening purposes and evaluating environmental exposure to Butocarboxim. (C) 2014 Elsevier Ltd. All rights reserved.
分类号: TS2`TS201.2
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