Development of a reverse genetics system based on RNA polymerase II for Newcastle disease virus genotype VII

文献类型： 外文期刊

第一作者： Wang, Jianzhong

作者： Wang, Jianzhong;Yin, Renfu;Cong, Yanlong;Ding, Zhuang;Wang, Chunfeng;Feng, Na;Wang, Hualei;Zheng, Xuexing;Yang, Songtao;Gao, Yuwei;Xia, Xianzhu;Liu, Xiufan;Hu, Shunlin;Ding, Chan;Yu, Shengqing

作者机构：

关键词： NDV;Genotype VII;Reverse genetics;RNA polymerase II

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Newcastle disease virus (NDV) has only a single serotype but diversified genotypes. Genotype VII strains are the prevalent currently circulating genotype worldwide, and in particular, these strains cause outbreaks in waterfowl. In this study, a reverse genetics system for highly virulent NDV isolated from goose flocks was developed independent of conventional T7 RNA polymerase. Infectious virus was successfully generated by an RNA polymerase II promoter to drive transcription of the full-length virus antigenome. A green fluorescent protein (GFP)-expressing virus was generated by inserting an additional transcription cassette coding for the enhanced GFP between the P and M genes of the genome. The expression of GFP was confirmed by western blotting and fluorescence microscopy. The replication kinetics and pathogenicity of the recombinant viruses are indistinguishable from the parental wild-type virus. This reverse genetics system will provide a powerful tool for the analysis of goose-origin NDV dissemination and pathogenesis, as well as preparation for genotype-matched NDV attenuated vaccines.

分类号： R37

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