The roles of Na+/K+-ATPase alpha-subunit gene from the ridgetail white prawn Exopalaemon carinicauda in response to salinity stresses

文献类型： 外文期刊

第一作者： Li, Jitao

作者： Li, Jitao;Ma, Peng;Liu, Ping;Chen, Ping;Li, Jian

作者机构：

关键词： Exopalaemon carinicauda;Na+/K+-ATPase alpha-subunit;proPO;Salinity adaptation;Expression

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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收录情况： SCI

摘要： Na+/K+-LATPase (NAK) is one important transporter protein and plays a key role in maintaining osmotic homeostasis in low and high salinity acclimation in variety of crustacean species. The ridgetail white prawn Exopalaemon carinicauda is an euryhaline and economic shrimp species in China, but it remains unclear about its mechanism of salinity adaption. In this study, a full-length of Na+/K+-ATPase alpha-subunit (alpha-NAK) cDNA was cloned from E. carinicauda by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of alpha-NAK was of 3680 bp, containing an open reading frame (ORF) of 3030 bp encoding a polypeptide of 1009 amino acids with the predicted molecular weight of 112.27 kDa. Eight transmembrane domains and two sites of phosphorylation and ATP binding were identified in E. carinicauda alpha-NAK. BLAST analysis revealed that the sequence of alpha-NAK amino acids of E. carinicauda shared more than 75% homologies with those of other crustacean. Real time quantitative RT-PCR analysis indicated that E. carinicauda alpha-NAK gene could be detected in all the tested tissues with highest expression level in gill. The expression profiles of E. carinicauda alpha-NAK transcripts were analyzed in gill and hepatopancreas tissues after salinity stresses. The results showed that the expression level of E. carinicauda alpha-NAK gene in both gill and hepatopancreas reached peak at different time after low and high salinity stresses, and showed different expression profiles. The expression profiles of proPO transcripts in gills after salinity stresses also indicated alpha-NAK and proPO played synergistic actions for salinity responses in E. carinicauda. These results indicated that E. carinicauda alpha-NAK involved in stress responses against salinity. (C) 2014 Elsevier Ltd. All rights reserved.

分类号： S9

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