Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells

文献类型： 外文期刊

第一作者： Ou, Xiuyuan

作者： Ou, Xiuyuan;Zhang, Xumeng;He, Qiyang;Ou, Xiuyuan;Zhang, Xumeng;He, Qiyang;Ou, Xiuyuan;Chen, Yan;Chen, Yan;Cheng, Xinxin;Cheng, Xinxin

作者机构：

关键词： hepatocellular carcinoma;resveratrol;matrine;apoptosis

期刊名称：ONCOLOGY REPORTS （ 影响因子：3.906； 五年影响因子：4.011 ）

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年卷期：

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收录情况： SCI

摘要： Resveratrol, a natural polyphenolic phytochemical, has received considerable attention due to its potential chemopreventive and chemotherapeutic properties. In the present study, we first evaluated the growth-inhibitory effect of resveratrol on HepG2 cells and explored the underlying molecular mechanisms. Resveratrol inhibited proliferation and induced apoptosis in HepG2 cells via activation of caspase-9 and caspase-3, upregulation of the Bax/Bcl-2 ratio and induction of p53 expression. Cell cycle analysis demonstrated that resveratrol arrested cell cycle progression in the G, and S phase. We further focused on the combination of matrine, a natural component extracted from the traditional Chinese medical herb Sophora flavescens Ait., as a mechanism to potentiate the growth-inhibitory effect of resveratrol on HepG2 cells. Both MTT and colony formation assay results indicated that the combined treatment of resveratrol and matrine exhibited a synergistic antiproliferative effect. In addition, resveratrol-induced apoptosis was significantly enhanced by matrine, which could be attributed to activation of caspase-3 and caspase-9, downregulation of survivin, induction of reactive oxygen species (ROS) generation and disruption of mitochondria membrane potential (Delta psi(m)). Our findings suggest that the combination treatment of resveratrol and matrine is a promising novel anticancer strategy for liver cancer; it also provides new insights into the mechanisms of combined therapy.

分类号： R73

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