Single-chain anti-idiotypic antibody retains its specificity to porcine reproductive and respiratory syndrome virus GP5

文献类型： 外文期刊

第一作者： Yu, Ying

作者： Yu, Ying;Li, Qiongyi;Du, Yongkun;Du, Taofeng;Mu, Yang;Xiao, Shuqi;Zhao, Qin;Wang, Chengbao;Sun, Yani;Xu, Xingang;Zhou, En-Min;Yu, Ying;Li, Qiongyi;Du, Yongkun;Du, Taofeng;Mu, Yang;Xiao, Shuqi;Zhao, Qin;Wang, Chengbao;Sun, Yani;Xu, Xingang;Zhou, En-Min;Wang, Gang;Cai, Xuehui;Zhang, Gaiping;Hsu, Walter H.

作者机构：

关键词： Anti-idiotype;Single-chain antibody;PRRSV

期刊名称：IMMUNOLOGY LETTERS （ 影响因子：3.685； 五年影响因子：3.54 ）

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年卷期：

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收录情况： SCI

摘要： Monoclonal anti-idiotypic antibody (Mab2-5G2) raised against idiotypic antibodies to membrane glycoprotein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV). The variable regions of the heavy chain (VH) and light chain (VL) of Mab2-5G2 were cloned and connected with a (Gly(4)Ser)(3) linker. The recombinant scFv gene was cloned into the pEasy-E1 vector and expressed in E. coli as inclusion bodies. The expressed scFv-His proteins renatured in a pH and urea gradient buffer retained the same immunological properties as that of Mab2-5G2. Renatured scFv-His protein retained the same characteristics as that of Mab2-5G2 by recognizing and binding to Marc-145 cells. Furthermore, renatured scFv-His along with Mab2-5G2 were used to immunize rabbits to produce anti-anti-idiotypic antibodies (Ab3) that neutralized PRRSV infection of Marc-145 cells. These results demonstrated that the expressed scFv-His protein possessed the same characteristics of Mab2-5G2 and will be suitable for future investigations of Mab2-5G2 antibody structure and its ability to interact with potential PRRSV cellular receptor as well as immunological properties against PRRSV infection. (C) 2014 Elsevier B.V. All rights reserved.

分类号： R392

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