Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China

文献类型： 外文期刊

第一作者： Pei, Shi-Chun

作者： Pei, Shi-Chun;Zhang, Hong-Fu;Ji, Cheng;Pei, Shi-Chun;Zhen, Yu-Ping;Gao, Jian-Wei;Lee, Won-Jong;Chen, Cong;Zhang, Xuan-Zhe

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关键词： feed;zearalenone;PCR;ELISA;China;Fusarium

期刊名称：FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE （ 影响因子：3.407； 五年影响因子：3.35 ）

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收录情况： SCI

摘要： Screening of zearalenone (ZEN)-producing species and monitoring of ZEN in feed were performed by using anti-zearalenone monoclonal antibodies. ELISA recoveries of ZEN from corn distillers dried grains with solubles (DDGS) feed, corn feed, rice bran, soybean meal, wheat bran and rapeseed dregs were between 78.6% and 88.6%. ZEN recovery from culture media was 96.3% at the spiked level of 500 mu g/kg. Eighty-three samples of DDGS feed, corn feed and other fee ingredients were collected from 11 provinces of China and analysed for ZEN. Average ZEN levels were 563.4 mu g/kg for DDGS feed, 393.1 mu g/kg for corn feed and 65.7 mu g/kg for other feed ingredients. Eighteen Fusarium species such as Fusarium graminearum which could produce ZEN were isolated from corn feed and other feed ingredients.

分类号： Q

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