Effect of ochratoxin A and buthionine sulfoximine on proteome and ascorbate-glutathione cycle enzymes in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Hao, J. R.

作者： Hao, J. R.;Wang, Y.;Zhao, W. W.;Xu, W. T.;Luo, Y. B.;Wu, W. H.;Huang, K. L.;Xu, W. T.;Luo, Y. B.;Yang, Z. J.;Liang, Z. H.;Huang, K. L.;Yang, Z. J.;Wu, W. H.;Wang, Y.

作者机构：

关键词： ascorbate peroxidase;glutathione peroxidase;glutathione reductase;glutathione-S-transferase;monodehydroascorbate reductase

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, proteome and activities of glutathione (GSH)-related enzymes were investigated in detached leaves of Arabidopsis thaliana treated with ochratoxin A (OTA) alone or supplemented with buthionine sulfoximine (BSO, a specific inhibitor of the first step in GSH biosynthesis). A comparative proteomic study using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry (MALDI-TOF/TOF MS/MS) identified 12 differentially expressed proteins mainly involved in GSH metabolism, energy metabolism, sugar metabolism, and photosynthesis. The treatment with OTA significantly enhanced the activities of glutathione-S-transferase (GST) and glutathione reductase (GR) through up-regulating the corresponding genes (GSTF7, GR1), an the diminishing effect of BSO on them counteracted the results. However, both OTA and BSO decreased the activity of ascorbate peroxidase (APX), and OTA also decreased the monodehydroascorbate reductase (MDHAR) and glutathione peroxidase (GPX) activities. Briefly, the OTA-induced phytotoxicity to the A. thaliana detached leaves was increased slightly by addition of BSO, and the fluctuation in GSH synthesis, GSH metabolism and disorder of cellular metabolism happened.

分类号： Q94

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