Overexpression of an Aeluropus littoralis Parl. potassium transporter gene, AlHAK1, in cotton enhances potassium uptake and salt tolerance

文献类型： 外文期刊

第一作者： Liu, J. F.

作者： Liu, J. F.;Zhang, S. L.;Tang, H. L.;Dong, L. J.;Wu, L. Z.;Liu, L. D.;Che, W. L.

作者机构：

关键词： Cotton (Gossypium hirsutum L.);AlHAK1;Transgenic plants;K+ uptake;Salt tolerance

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

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收录情况： SCI

摘要： Potassium (K) deficiency and salinity are major environmental stresses affecting the growth and agricultural production of cotton (Gossypium hirsutum) plants worldwide. In an effort to improve potassium uptake and tolerance to salt stress through genetic engineering, we inserted AlHAK1 into cotton via Agrobacterium-mediated transformation, and then transgenic lines were obtained. To test their response to K-starvation and salt stress, we cultured seedlings from the wild type (WT) and three homozygous overexpression lines (T-3 generation) in plastic pots and treated them with a modified half-strength Hoagland's solution supplemented with different concentrations of potassium or sodium: NT (normal treatment with standard amounts of K and Na), KT (additional 0.05 mM KCl), ST (additional 150 mM NaCl), or KST (0.05 mM KCl plus 150 mM NaCl). After 15 days, all transgenic lines exhibited significantly larger values for shoot and root lengths and biomass (shoot dry weight or root dry weight) when compared with WT plants. Most root morphological parameters for the transgenics were also increased, e.g., total lengths, specific root lengths and surface areas. However, average root diameters were significantly lower than that of the WT (P < 0.05 or P < 0.01). Under salt-stress conditions, the ratios for K+/Na+ were higher in the leaves and roots of transgenic plants, and they also had less malondialdehyde and hydrogen peroxide (H2O2) than the WT tissues. Those responses paralleled greater activities by the antioxidant enzymes superoxide dismutase and peroxidase. We clearly demonstrated that cotton plants transformed with a high-affinity K+ transporter gene have enhanced K+ uptake and salt tolerance. These findings could serve as a promising step toward the development of new cotton cultivars with improved potassium uptake and tolerance to salt stress, and they have significant implications for increasing crop yields on high-salinity soils where potassium levels are low.

分类号： S3

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