Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique

文献类型： 外文期刊

第一作者： Sun, Dongbo

作者： Sun, Dongbo;Shi, Hongyan;Chen, Jianfei;Shi, Da;Zhang, Xin;Feng, Li;Sun, Dongbo;Guo, Donghua;Zhu, Qinghe

作者机构：

关键词： PEDV;Infection-associated proteins;Quantitative proteomics

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recent outbreaks of porcine epidemic diarrhea virus (PEDV) have caused widespread concern. The identification of proteins associated with PEDV infection might provide insight into PEDV pathogenesis and facilitate the development of novel antiviral strategies. We analyzed the differential protein profile of PEDV-infected Vero E6 cells using mass spectrometry and an isobaric tag for relative and absolute quantification. A total of 126 proteins were identified that were differentially expressed between the PEDV-infected and mock-infected groups (P<0.05, quantitative ratio >= 1.2), among which the expression of 58 proteins was up-regulated and that of 68 proteins was down-regulated in the PEDV-infected Vero E6 cells, involving in integrin beta 2/beta 3, cystatin-C. The Gene Ontology analysis indicated that the molecular function of the differentially expressed proteins (DEPs) was primarily related to binding and catalytic activity, and that the biological functions in which the DEPs are involved included metabolism, organismal systems, cellular processes, genetic information processing, environmental information processing, and diseases. Among the disease-related functions, certain anti-viral pathways and proteins, such as the RIG-I-like receptor, Ran autophagy, mitogen-activated protein kinase, PI3K-Akt and Jak-STAT signaling pathways, and integrin beta 2/beta 3 and cystatin-C proteins, represented potential factors in PEDV infection. Our findings provide valuable insight into PEDV-Vero E6 cell interactions. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R37

  • 相关文献

[1]Quantitative proteomics by amino acid labeling in foot-and-mouth disease virus (FMDV)-infected cells. Ye, Yu,Xin, Chaoan,Liao, Ming,Fan, Huiying,Ye, Yu,Luo, Yongwen,Xin, Chaoan,Liao, Ming,Fan, Huiying,Ye, Yu,Xin, Chaoan,Liao, Ming,Fan, Huiying,Tong, Tiezhu,Yan, Guangrong,Yan, Guangrong,Liu, Xiangtao.

[2]Comparative analysis of primary hepatocellular carcinoma with single and multiple lesions by iTRAQ-based quantitative proteomics. Xing, Xiaohua,Huang, Yao,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Jingfeng,Xing, Xiaohua,Wang, Sen,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Minjie,Liu, Xiaolong,Liu, Jingfeng,Xing, Xiaohua,Wang, Sen,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Minjie,Liu, Xiaolong,Liu, Jingfeng,Han, Xiao.

[3]Proteomic characterization and comparison of mammalian milk fat globule proteomes by iTRAQ analysis. Yang, Yongxin,Zheng, Nan,Zhao, Xiaowei,Zhang, Yangdong,Han, Rongwei,Ma, Lu,Zhao, Shengguo,Li, Songli,Guo, Tongjun,Wang, Jiaqi,Yang, Yongxin,Zhao, Xiaowei,Zheng, Nan,Zhang, Yangdong,Zhao, Shengguo,Li, Songli,Wang, Jiaqi,Han, Rongwei.

[4]Proteome Differences between Hepatitis B Virus Genotype-B- and Genotype-C-Induced Hepatocellular Carcinoma Revealed by iTRAQ-Based Quantitative Proteomics. Wei, Dahai,Zeng, Yongyi,Xing, Xiaohua,Liu, Hongzhi,Lin, Minjie,Liu, Xiaolong,Liu, Jingfeng,Wei, Dahai,Zeng, Yongyi,Xing, Xiaohua,Liu, Hongzhi,Lin, Minjie,Liu, Xiaolong,Liu, Jingfeng,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao,Liu, Xiaolong,Liu, Jingfeng.

[5]Reveal the molecular signatures of hepatocellular carcinoma with different sizes by iTRAQ based quantitative proteomics. Wang, Yingchao,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Xing, Xiaohua,Xia, Jiangbao,Lin, Minjie,Liao, Naishun,Liu, Xiaolong,Liu, Jingfeng,Wang, Yingchao,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Xing, Xiaohua,Xia, Jiangbao,Lin, Minjie,Liao, Naishun,Liu, Xiaolong,Liu, Jingfeng,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao.

[6]Differential proteomic analysis of rice seedlings reveals the advantage of dry-raising nursery practices. Zhang, Zhixing,Huang, Fenglian,Chen, Hongfei,Lin, Wenxiong,Zhang, Zhixing,Huang, Fenglian,Chen, Hongfei,Lin, Wenxiong,Shao, CaiHong. 2018

[7]Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann. Ji, Ting,Liu, Zhenguo,Shen, Jie,Shen, Fang,Chen, Guohong,Liang, Qin,Wu, Liming,Corona, Miguel. 2014

[8]Proteomic analysis of sheep primary testicular cells infected with bluetongue virus. Du, Junzheng,Xing, Shanshan,Tian, Zhancheng,Gao, Shandian,Xie, Junren,Chang, Huiyun,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[9]Comparative Proteomic Analysis of Wild-Type and SAP Domain Mutant Foot-and-Mouth Disease Virus-Infected Porcine Cells Identifies the Ubiquitin-Activating Enzyme UBE1 Required for Virus Replication. Zhu, Zixiang,Yang, Fan,Zhang, Keshan,Cao, Weijun,Jin, Ye,Wang, Guoqing,Mao, Ruoqing,Li, Dan,Guo, Jianhong,Liu, Xiangtao,Zheng, Haixue.

[10]Quantitative proteomics of Sesuvium portulacastrum leaves revealed that ion transportation by V-ATPase and sugar accumulation in chloroplast played crucial roles in halophyte salt tolerance. Yi, Xiaoping,Sun, Yong,Yang, Qian,Guo, Anping,Chang, Lili,Wang, Dan,Tong, Zheng,Jin, Xiang,Wang, Limin,Wang, Xuchu,Yi, Xiaoping,Guo, Anping,Chang, Lili,Wang, Xuchu,Yu, Jianlan,Jin, Wenhai.

[11]Proteomic analysis of conidia germination in Fusarium oxysporum f. sp cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Deng, Gui-Ming,Yang, Qiao-Song,He, Wei-Di,Li, Chun-Yu,Yang, Jing,Zuo, Cun-Wu,Gao, Jie,Sheng, Ou,Yi, Gan-Jun,Deng, Gui-Ming,Yang, Qiao-Song,He, Wei-Di,Li, Chun-Yu,Yang, Jing,Zuo, Cun-Wu,Gao, Jie,Sheng, Ou,Yi, Gan-Jun,Deng, Gui-Ming,Yang, Jing,Zuo, Cun-Wu,Gao, Jie,Lu, Shao-Yun,Yi, Gan-Jun,He, Wei-Di,Zhang, Sheng.

[12]iTRAQ, analysis of the tobacco leaf proteome reveals that RNA-directed DNA methylation (RdDM) has important roles in defense against geminivirus-betasatellite infection. Wang, Zhan Qi,Xiao, Ruyuan,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Zhou, Xueping.

[13]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[14]Detection and phylogenetic analyses of spike genes in porcine epidemic diarrhea virus strains circulating in China in 2016-2017. Zhang, Qiaoling,Liu, Xinsheng,Fang, Yuzhen,Zhou, Peng,Wang, Yonglu,Zhang, Yongguang,Zhang, Qiaoling,Liu, Xinsheng,Fang, Yuzhen,Zhou, Peng,Wang, Yonglu,Zhang, Yongguang. 2017

[15]Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene. Su, Yunfang,Ji, Pengchao,Liu, Yunchao,Chen, Yumei,Xing, Guangxu,Liu, Chang,Deng, Ruiguang,Zhang, Gaiping,Zhao, Baolei,Jiang, Dawei,Song, Yapeng,Wang, Guoqiang,Li, Dongliang,Zhang, Gaiping,Zhang, Gaiping. 2016

[16]PEDV ORF3 encodes an ion channel protein and regulates virus production. Wang, Kai,Lu, Wei,Xie, Shiqi,Yu, Wenjing,Feng, Li,Sun, Bing,Bian, Chao,Sun, Bing,Chen, Jianfei,Shi, Hongyan,Feng, Li,Hsu, Haojen,Fischer, Wolfgang B.,Schwarz, Wolfgang,Schwarz, Wolfgang.

[17]Tight Junction Protein Occludin Is a Porcine Epidemic Diarrhea Virus Entry Factor. Luo, Xiaolei,Guo, Longjun,Zhang, Jian,Xu, Yunfei,Gu, Weihong,Feng, Li,Wang, Yue.

[18]Outbreak of Porcine Epidemic Diarrhea in Piglets in Gansu Province, China. Wang, Hui,Xia, Xinchao,Liu, Yongming,Wang, Shengyi,Qi, Zhiming,Liu, Shixiang,Wang, Haijun,Liu, Zhiqi,Niu, Xu,Liu, Shanshan. 2013

[19]Sequence Heterogeneity of the ORF3 Gene of Porcine Epidemic Diarrhea Viruses Field Samples in Fujian, China, 2010-2012. Chen, Xi,Yang, Jinxian,Yu, Fusong,Ge, Junqing,Gao, Xindang,Song, Tieying,Zeng, Lili,Guo, Qing. 2013

[20]Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China. Chen, Jianfei,Liu, Xiaozhen,Shi, Da,Shi, Hongyan,Zhang, Xin,Li, Changlong,Chi, Yanbin,Feng, Li. 2013

作者其他论文 更多>>

微信小程序