Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods

文献类型: 外文期刊

第一作者: Ringo, Einar

作者: Ringo, Einar;Andersen, Renate;Breines, Eva Marie;Hareide, Ellinor;Yttergard, Gerd Jorun;Opsal, Kirstin;Johansen, Heidi Mathilde;Andreassen, Ann Kristin;Kousha, Armin;Godfroid, Jacques;Sperstad, Sigmund;Zhou, Zhigang;Ren, Pen;Holzapfel, Wilhelm

作者机构:

关键词: 16S rRNA clone library;fermented horse milk;bacterial community;cultivation;denaturing gradient gel electrophoresis

期刊名称:FOOD BIOTECHNOLOGY ( 影响因子:1.564; 五年影响因子:1.521 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The bacterial community of fermented horse milk (koumiss) from Mongolia was studied using three methods: cultivation, direct identification by 16S rRNA clone library and denaturing gradient gel electrophoresis (DGGE). Ninety-eight strains were isolated by traditional cultivation and 61 of those were randomly selected for further identification by 16S rRNA gene sequencing. The strains were dominated by lactic acid bacteria (LAB; six different lactobacilli), Acinetobacter, Bacillus and Psychrobacter. Construction of the clone library analysis revealed that 16S sequences of 220 clones, genus Lactobacillus was dominant, but Streptococcus thermophilus, Acetobacter pasteurianus and uncultured clones were also detected. Ten unique bands were sequenced from the DGGE and revealed: Lactococcus lactis, Lactococcus lactis subsp. lactis, Clostridium acidurici, Acinetobacter johnsonii, Dickeya sp., Enterobacter sp., Pseudomonas sp., Raoultella sp., and Ruminococcus sp.. In vitro growth inhibition of three human pathogens, Escherichia coli, Enterobacter sakazakii and Staphylococcus aureus by 14 culturable bacteria displayed that only three of the isolates tested inhibit growth of E. sakazakii while most of the other bacteria delayed growth of the target bacteria.

分类号: TS2

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