Identification and examination of a novel 9-bp insert/deletion polymorphism on porcine SFTPA1 exon 2 associated with acute lung injury using an oleic acid-acute lung injury model

文献类型： 外文期刊

第一作者： Zhang, Yuebo

作者： Zhang, Yuebo;Zhang, Longchao;Wang, Ligang;Liang, Jing;Yan, Hua;Zhao, Kebin;Liu, Xin;Wang, Lixian;Qiao, Lijuan

作者机构：

关键词： acute lung injury;indel polymorphism;OA-ALI model;pig;SFTPA1 gene

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.749； 五年影响因子：1.909 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The pulmonary surfactant-associated protein (SFTPA1, SP-A) gene has been studied as a candidate gene for lung disease resistance in humans and livestock. The objective of the present study was to identify polymorphisms of the porcine SFTPA1 gene coding region and its association with acute lung injury (ALI). Through DNA sequencing and the PCR-single-strand conformation polymorphism method, a novel 9-bp nucleotide insertion (+) or deletion (-) was detected on exon 2 of SFTPA1, which causes a change in three amino acids, namely, alanine (Ala), glycine (Gly) and proline (Pro). Individuals of three genotypes (-/-, +/- and +/+) were divided into equal groups from 60 Rongchang pigs that were genotyped. These pigs were selected for participation in the oleic acid (OA)-ALI model by 1-h and 3-h injections of OA, and there were equal numbers of pigs in the control and injection groups. The lung water content, a marker for acute lung injury, was measured in this study; there is a significant correlation between high lung water content and the presence of the 9-bp indel polymorphism (P<0.01). The lung water content of the OA injection group was markedly higher than that of the control group and lung water content for the +/+ genotype was significantly higher than that of the others in the 1-h group (P<0.01). No differences in the expression of the SFTPA1 gene were found among individuals with different SFTPA1 genotypes, indicating that the trait is not caused by a linked polymorphism causing altered expression of the gene. The individuals with the -/- genotype showed lower lung water content than the +/+ genotype pigs, which suggests that polymorphism could be a potential marker for lung disease-resistant pig breeding and that pig can be a potential animal model for human lung disease resistance in future studies.

分类号： S8

  • 相关文献

[1]Callicarpa japonica Thunb. reduces inflammatory responses: A mouse model of lipopolysaccharide-induced acute lung injury. Shin, Na-Rae,Shin, In-Sik,Song, Hyuk-Hwan,Hong, Ju-Mi,Kwon, Ok-Kyoung,Jeon, Chan-Mi,Kim, Jung-Hee,Oh, Sei-Ryang,Ahn, Kyung-Seop,Shin, Na-Rae,Hahn, Kyu-Woung,Shin, In-Sik,Lee, Sang-Woo,Lee, Joong-Ku,Jin, Hang,Li, Wan Yi. 2015

[2]Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-kappa B activation. Zhao, Zhanzhong,Tang, Xiangfang,Zhang, Minhong,Hou, Shaohua,Yuan, Weifeng,Zhang, Hongfu,Shi, Lijun,Jia, Hong,Liang, Lin,Zhao, Zhanzhong,Zhao, Xinghui,Fu, Ling,Chen, Wei,Zhang, Weijian,Lai, Zhi,Gao, Junfeng,Zhang, Keyu. 2014

[3]Ultrastructural changes in the pulmonary mechanical barriers in a rat model of severe acute pancreatitis-associated acute lung injury. Wang, Feng,Lu, Fengchun,Huang, Heguang,Huang, Minmin,Luo, Tuyan.

[4]Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2 '-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Liu, Shi Chao,Zhou, Yang,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,He, Hong Bin. 2013

[5]Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Li, Jing Yu,Xue, Bing Hua,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,Wu, Zhan Feng,Zhang, Ji Guang.

[6]A genome-wide detection of copy number variations using SNP genotyping arrays in swine. Wang, Jiying,Jiang, Jicai,Fu, Weixuan,Jiang, Li,Ding, Xiangdong,Liu, Jian-Feng,Zhang, Qin,Wang, Jiying. 2012

[7]Single nucleotide polymorphism scanning and expression of the FRZB gene in pig populations. Wang, Zhixiu,Zhang, Bo,Yang, Yuzeng,Ban, Dongmei,Zhang, Hao,Li, Qinggang,Lu, Yunfeng. 2014

[8]Effects of Monoclonal Antibody Against Porcine 40-kDa Adipocyte-Specific Membrane Protein on Endocrine Secretion in Pigs. Zhao Su-mei,Zhang Xi,Duan Gang,Gao Shi-zheng,Hu Hong-mei,Liu Ling-yun. 2009

[9]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[10]Molecular cloning, sequence analysis, and function of the intestinal epithelial stem cell marker Bmi1 in pig intestinal epithelial cells. Li, C. -M.,Yan, H. -C.,Fu, H. -L.,Xu, G. -F.,Wang, X. -Q.. 2014

[11]Wnt antagonist, secreted frizzled-related protein 1, is involved in prenatal skeletal muscle development and is a target of miRNA-1/206 in pigs. Yang, Yalan,Sun, Wei,Wang, Ruiqi,Zhou, Rong,Tang, Zhonglin,Li, Kui,Yang, Yalan,Tang, Zhonglin,Li, Kui,Sun, Wei,Lei, Chuzhao. 2015

[12]Integrated analysis of mRNA and miRNA expression profiles in livers of Yimeng black pigs with extreme phenotypes for backfat thickness. Li, Wentong,Yang, Yalan,Zhou, Rong,Li, Kui,Li, Wentong,Liu, Shuai,Li, Xiuxiu,Wang, Yingping,Tang, Hui,Li, Wentong,Yang, Yalan,Liu, Ying,Zhang, Yanmin,Zhou, Rong,Li, Kui,Li, Kui. 2017

[13]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[14]Identification and genetic effect of haplotypes in the promoter region of porcine myostatin gene. Liu, D.,Xu, Q.,Zang, L.,Liang, S.,Jiang, Y.,Wu, Y.,Wei, S.. 2011

[15]Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds. Li, Qinggang,Lu, Yunfeng. 2014

[16]A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs. Liu Yonggang,Pu Shifei,Kong Chuisi. 2012

[17]A SOLUTION ON IDENTIFICATION AND REARING FILES IN SMALLHOLD PIG FARMING. Xiong, Benhai,Luo, Qingyao,Yang, Liang,Xiong, Benhai,Luo, Qingyao,Yang, Liang,Fu, Runting,Lin, Zhaohui. 2009

[18]Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs. Wang, Yuding,Ma, Cai,Sun, Yi,Kang, Li,Jiang, Yunliang,Wang, Yuding,Li, Yi. 2017

[19]Molecular cloning, expression and variation analyses of the dopamine D2 receptor gene in pig breeds in China. Xu, H. P.,He, X. M.,Hu, Y. S.,Jia, X. Z.,Nie, Q. H.,Zhang, X. Q.,Xu, H. P.,He, X. M.,Hu, Y. S.,Jia, X. Z.,Nie, Q. H.,Zhang, X. Q.,Fang, M. X.. 2011

[20]Selection of reference genes for studies of porcine endometrial gene expression on gestational day 12. Zhang, Hao. 2011

作者其他论文 更多>>

微信小程序