A simple pollen collection, dehydration, and long-term storage method for litchi (Litchi chinensis Sonn.)

文献类型： 外文期刊

第一作者： Wang, Limin

作者： Wang, Limin;Wu, Jiefang;Chen, Jiezhen;Fu, Danwen;Zhang, Chunyang;Cai, Changhe;Ou, Liangxi;Wang, Limin;Wu, Jiefang;Chen, Jiezhen;Fu, Danwen;Zhang, Chunyang;Cai, Changhe;Ou, Liangxi

作者机构：

关键词： Litchi;Anther;Desiccation;Cryopreservation;Germination;Crossbreeding

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A simple method for pollen collection and storage was devised for three litchi cultivars, namely, 'Sanyuehong,'Shuidong,' and 'Guiwei'. This method could realize long-term pollen storage over periods of up to two years for artificial pollination and crossbreeding. The most suitable drying method for litchi pollen was the use of an air-blowing electric dryer at 35 degrees C for 6 h. The germination rate of pollen stored at different temperatures (30 degrees C, 25 degrees C, 15 degrees C, 4 degrees C, and 86 degrees C) was investigated. The pollen germination rate decreased to lower than 5% after 9, 16, 27, and 52 d at 30 degrees C, 25 degrees C, 15 degrees C, and 4 degrees C, respectively. A significant negative correlation was observed between pollen germination rate and storage time. For all litchi cultivars tested, pollen cryo-stored (-86 C) for one year showed significantly higher germination rates than those stored under the other conditions. Pollen stored at 86 C maintained a high germination rate of above 70% even after two years of storage. Thus, our results indicated that storage of pollen at 4 degrees C was suitable for field pollinations in the blooming season for up to two months. However, storage at 86 degrees C maintained the high germination rates required for artificial hybridization even in the second year of storage. (C) 20115 Elsevier B.V. All rights reserved.

分类号： S6

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