Genetic analysis and identification of major locus and candidate genes linked to thousand-seed weight in flax (Linum usitatissimum)

文献类型： 外文期刊

第一作者： Qi, Yanni

作者： Qi, Yanni;Wang, Limin;Li, Wenjuan;Dang, Zhao;Zhao, Wei;Wang, Ping;Xie, Yaping;Xu, Chenmeng;Wang, Yan;Zhang, Jianping;Wang, Wei

作者机构：

关键词： Flax; TSW; BSA-seq; Fine mapping; qRT-PCR

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 230 卷

页码：

收录情况： SCI

摘要： Flax (Linum usitatissimum) is a valuable oilseed and cash crop with multiple applications as food, animal feed, and medicine, in addition to its industrial applications. Thousand-seed weight (TSW) is one of the most critical components of seed yield in flax, and breeding efforts have been directed toward obtaining flax varieties with high TSW. However, the genes associated with TSW in flax remain understudied. This study investigated the major locus and genes linked to TSW in flax through a comprehensive analysis, encompassing bulked segregant analysis, fine mapping, qRT-PCR, and functional annotations. Herein, we performed a genetic analysis of the F2 and F2:3 populations from our previous study, which indicated that TSW is controlled by multiple genes. Bulked segregant analysis revealed a major quantitative trait locus linked to TSW, namely TSW3.1, which is located on Chromosome 3. Subsequently, TSW3.1 was fine-mapped to a 23.38-kb interval comprising 5 genes through the linkage mapping analysis. Four of the five genes exhibited variations in either their coding regions or their upstream/downstream regulatory regions. Based on the qRT-PCR analysis and gene annotation, all five genes were identified as the candidates for TSW. This study offers fresh insights into the genetic mechanisms of TSW regulation in flax, and the findings may serve as reference for breeding new varieties with high TSW.

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