Characterization of two pigeon paramyxovirus type 1 isolates in China

文献类型： 外文期刊

第一作者： Awu, Abie

作者： Awu, Abie;Shao, Meng-yu;Liu, Meng-meng;Hu, Yan-xin;Zhang, Guo-zhong;Qin, Zhuo-ming;Tian, Fu-lin

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期刊名称：AVIAN PATHOLOGY （ 影响因子：3.378； 五年影响因子：3.237 ）

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收录情况： SCI

摘要： For over three decades, there has been a continuing panzootic caused by a virulent variant avian paramyxovirus type 1 strain, the so-called pigeon paramyxovirus type 1. It is found primarily in racing pigeons, but it has also spread to wild birds and poultry. In this study, two pigeon paramyxovirus type 1 strains, SD12 and BJ13, obtained from diseased pigeons in China, were characterized. Phylogenetic analysis based on complete sequences allowed characterization of both strains as genotype VI, class II. Further phylogenetic analysis of a 374-nucleotide section of the fusion gene showed that SD12 fell into lineage VIbii-d and BJ13 into VIbii-f. The deduced amino acid sequence of the cleavage site of the fusion protein confirmed that both isolates contained the virulent motif K-112/RRQKR down arrow F-117 at the cleavage site. Nevertheless, the values of intracerebral pathogenicity indices showed the SD12 isolate to be a velogenic strain and BJ13 isolate to be a mesogenic strain. The SD12 isolate was further investigated via clinical observation, RNA detection, histopathology and viral serology in experimentally infected 3-week-old chickens. It showed a mild pathological phenotype in chickens, with viral replication restricted to a few tissues. The molecular mechanism for the SD12 isolate to have a virulent motif but low levels of virulence for chickens requires further study.

分类号： R36

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