Pathotypical characterization and molecular epidemiology of Newcastle disease virus isolates from different hosts in China from 1996 to 2005

文献类型： 外文期刊

第一作者： Qin, Zhuo-Ming

作者： Qin, Zhuo-Ming;Tan, Lei-Tao;Xu, Huai-Ying;Ma, Bao-Chen;Wang, You-Ling;Yuan, Xiao-Yuan;Liu, Wen-Jun

作者机构：

期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.948； 五年影响因子：5.345 ）

ISSN： 0095-1137

年卷期： 2008 年 46 卷 2 期

页码：

收录情况： SCI

摘要： Thirty Newcastle disease virus (NDV) strains isolated from outbreaks in China during 1996 to 2005 were characterized pathotypically and genotypically. All strains except one were velogenic. An analysis of the variable region (nucleotides 47 to 420) of the F gene indicated that 6 isolates belonged to genotype 11, 3 to genotype 111, 1 (isolated from a pigeon) to genotype VI, and 20 to genotype VII. Isolates belonging to genotype VII were further divided into five subtypes, VIIa, VIIb, VIIc, VIId, and VIIe, and subtype VIId was made up of VIM to VIId5. These results showed that genotype VII isolates might have been the most prevalent in China during the past two decades. Genotype VII isolates shared high homology, but the homology was less than that between genotype VII viruses and the vaccine virus LaSota. Among these NDV isolates, 25 isolates had the velogenic Motif R-112/K-R-Q-K/R-R-F-117 that is consistent with results of the biological tests. However, four of five LaSota-type isolates that contained the lentogenic Motif (112)G-R-Q-G-R-L-117 were velogenic, except SY/03, in the view of the biological test. The majority of genotype VII isolates had lost one or two N-glycosylation sites. Finally, a cross-protection experiment in which specific-pathogen-free chickens vaccinated with LaSota were challenged by six NDV isolates showed that more than three isolates were antigenic variants that could be responsible for recent outbreaks of Newcastle disease.

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