The effect of heat stress on proliferation, synthesis of steroids, and gene expression of duck granulosa cells

文献类型： 外文期刊

第一作者： Yang, Chen

作者： Yang, Chen;Huang, Xue-bing;Chen, Shi-jian;Li, Xiu-jin;Fu, Xin-liang;Xu, Dan-ning;Tian, Yun-bo;Liu, Wen-jun;Huang, Yun-mao;Yang, Chen;Huang, Xue-bing;Chen, Shi-jian;Li, Xiu-jin;Fu, Xin-liang;Xu, Dan-ning;Tian, Yun-bo;Liu, Wen-jun;Huang, Yun-mao;Huang, Xue-bing

作者机构：

关键词： duck; granulosa cells; heat stress; transcriptome

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：1.749； 五年影响因子：1.909 ）

ISSN： 1344-3941

年卷期： 2021 年 92 卷 1 期

页码：

收录情况： SCI

摘要： Granulosa cells (GCs) play an important role in the development of follicles. In this study, we investigate the impact of heat stress at 41 degrees C and 43 degrees C on duck GCs' proliferation and steroids secretion. And, the transcriptomic responses to heat treatment were examined using RNA-sequencing analysis. Digital gene expression profiling was used to screen and identify differentially expressed genes (fold change >= 2 and Q value < 0.05). Further, the differential expression genes (DEGs) were classified into GO categories and KEGG pathways. The results show that duck GCs blocked in the G1 phase were increased on exposure to heat stress. Meanwhile, the expression of proliferative genes, which were essential for the transition from G1 to S phase, was inhibited. At the same time, heat stress inhibited the estradiol synthesis of GCs by decreasing CYP11A1 and CYP19A1 gene expression. A total of 241 DEGs including 181 upregulated and 60 downregulated ones were identified. Transcriptome result shows that heat shock protein and CXC chemokines gene were significantly activated during heat stress. While collagenases (MMP1 and MMP13) and strome lysins (MMP3) were downregulated. And, the hedgehog signaling pathway may be a prosurvival adaptive response under heat stress. These results offer a basis for better understanding the molecular mechanism underlying lay-eggs-less in ducks under heat stress.

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