Real Time PCR Quantification of Sclerotium rolfsii in Chilli Tissue and Soil

文献类型： 外文期刊

第一作者： Guo, Rong-Jun

作者： Guo, Rong-Jun;Li, Shi-Dong;Miao, Wu

作者机构：

关键词： chilli root rot;specific primer pair;pathogen identification;population detection;population determination

期刊名称：PLANT PROTECTION SCIENCE （ 影响因子：1.464； 五年影响因子：1.834 ）

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收录情况： SCI

摘要： Root rot disease caused by Sclerotium rolfsii is one of the important factors limiting chilli production in southern China. Rapid methods of detection and identification of S. rolfsii are required for disease prediction and management. Herein, we adapted a four-step real time quantitative PCR method to detect S. rolfsii using new designed primer pair S301S/S301A. By using this assay, S. rolfsii was detected in ten soil samples and eight plant samples at concentrations as low as 10(-7) g mycelium/g soil or plant, both with and without typical symptoms, while tissue-culturing method could only detect the fungus in two samples. This rapid and quantitative method is efficient for detection and determination of S. rolfsii population in soil and plants.

分类号： S4

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