Replacing antibodies with aptamers in lateral flow immunoassay

文献类型： 外文期刊

第一作者： Chen, Ailiang

作者： Chen, Ailiang;Yang, Shuming;Chen, Ailiang;Yang, Shuming

作者机构：

关键词： Aptamer;Antibody;Lateral flow;Immunochromatography;Strip test

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aptamers have been identified against various targets as a type of chemical or nucleic acid ligand by systematic evolution of ligands by exponential enrichment (SELEX) with high sensitivity and specificity. Aptamers show remarkable advantages over antibodies due to the nucleic acid nature and target-induced.structure-switching properties and are widely used to design various fluorescent, electrochemical, or colorimetric biosensors. However, the practical applications of aptamer-based sensing and diagnostics are still lagging behind those of antibody-based tests. Lateral flow imthunoassay (LFIA) represents a well established and appropriate technology among rapid assays because of its low cost and user-friendliness. The antibody-based platform is utilized to detect numerous targets, but it is always hampered by the antibody preparation time, antibody stability, and effect of modification on the antibody. Seeking alternatives to antibodies is an area of active research and is of tremendous importance. Aptamers are receiving increasing attention in lateral flow applications because of a number of important potential performance advantages. We speculate that aptamer-based LFIA may be one of the first platforms for commercial use of aptamer-based diagnosis. This review first gives an introduction to aptamer including the selection process SELEX with its focus on aptamer advantages over antibodies, and then depicts LFIA with its focus on aptamer opportunities in LFIA over antibodies. Furthermore, we summarize the recent advances in the development of aptamer-based lateral flow biosensing assays with the aim to provide a general guide for the design of aptamer-based lateral flow biosensing assays. (C) 2015 Elsevier B.V. All rights reserved.

分类号： Q

  • 相关文献

[1]Dual-competitive lateral flow aptasensor for detection of aflatoxin B-1 in food and feedstuffs. Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yang, Shuming,Chen, Ailiang,Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yang, Shuming,Chen, Ailiang,Huang, Yafei,Ren, Shuyue,Gao, Zhixian. 2018

[2]Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers. Xu, Lin,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Xu, Lin,Li, Peiwu,Xu, Lin,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhang, Qi,Li, Peiwu,Li, Peiwu. 2016

[3]Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber. Yang, Xingdong,Zeng, Xianyin,Yang, Xingdong,Zhang, Gaiping,Wang, Fangyu,Wang, Yinbiao,Hu, Xiaofei,Li, Qingmei,Jia, Guochao,Liu, Zhen,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping.

[4]Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions. Yin, Fangyuan,Liu, Junlong,Liu, Aihong,Li, Youquan,Luo, Jianxun,Guan, Guiquan,Yin, Hong,Yin, Hong.

[5]Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus. Yang, Yang,Qin, Xiaodong,Wang, Guangxiang,Jin, Jiaxin,Shang, Youjun,Zhang, Zhidong,Jin, Jiaxin. 2016

[6]Current development of microfluidic immunosensing approaches for mycotoxin detection via capillary electromigration and lateral flow technology. Li, Peiwu,Zhang, Zhaowei,Zhang, Qi,Zhang, Ning,Zhang, Wen,Ding, Xiaoxia,Li, Ran,Li, Peiwu,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhang, Zhaowei,Zhang, Ning,Li, Peiwu,Ding, Xiaoxia,Li, Peiwu,Zhang, Wen,Li, Ran.

[7]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[8]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[9]Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus. Li, Huawei,Ji, Pengchao,Zhou, Enmin,Zhang, Gaiping,Yang, Jifei,Bao, Dengke,Hou, Jie,Zhi, Yubao,Yang, Yanyan,Qiao, Songlin,Zhang, Gaiping,Zhang, Gaiping,Li, Huawei.

[10]'Green' immunochromatographic electrochemical biosensor for mercury(II). Wang, Yulong,Wang, Limin,Wang, Suyan,Yang, Mingming,Cai, Jia,Liu, Fengquan,Liu, Fengquan.

[11]Antibody Developments and Immunoassays for Organophosphorus Chemicals: A Review. Hua, Xiude,Liu, Fengquan,Wang, Minghua,Hua, Xiude,Liu, Fengquan,Wang, Minghua,Eremin, Sergei A.,Eremin, Sergei A.,Liu, Fengquan. 2017

[12]Development of Monoclonal Antibodies Recognizing Linear Epitope: Illustration by Three Bacillus thuringiensis Crystal Proteins of Genetically Modified Cotton, Maize, and Tobacco. Cao, Zhen,Zhang, Wei,Ning, Xiangxue,Wang, Baomin,Cao, Zhen,Li, Qing X.,Liu, Yunjun. 2017

[13]Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Zhang, WY,Moreau, E,Yang, BZ,Li, ZQ,Hope, JC,Howard, CJ,Huang, WY,Chauvin, A. 2006

[14]Developments on Immunoassays for Pyrethroid Chemicals. Jin, Maojun,Chen, Ge,Du, Pengfei,Zhang, Chan,Cui, Xueyan,She, Yongxin,Zheng, Lufei,Wang, Shanshan,Shao, Hua,Jin, Fen,Wang, Jing,Gee, Shirley J.,Hammock, Bruce D.,Gee, Shirley J.,Hammock, Bruce D.. 2017

[15]Leptin receptor signaling inhibits ovarian follicle development and egg laying in chicken hens. Lei, Ming M.,Chen, Zhe,Shi, Zhen D.,Wu, Si Q.,Li, Xiao W.,Wang, Cong L.. 2014

[16]Synthesis and identification of the ciprofloxacin hapten-protein Conjugates. Huang, Xuan-yun,Huang, Dong-mei,Shi, Yong-fu,Feng, Bing,Yu, Hui-juan,Cai, You-qiong. 2013

[17]Effects of dietary vitamin E on immunological stress of layers and their offspring. Zhao, G. P.,Han, M. J.,Zheng, M. Q.,Zhao, J. P.,Chen, J. L.,Wen, J..

[18]Immunoassays for aflatoxins. Li, Peiwu,Zhang, Qi,Zhang, Wen.

[19]A method to label biological molecules with dsDNA coated gold nanoparticles. Xing, Ming,Dong, Yingshan,Xing, Ming,Li, Fangliang,Chen, Xia,Xing, Ming,Li, Fangliang,Chen, Xia,Yu, Zhenxiang.

[20]A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum. Sun, Yun,Sun, Li,Liu, Chun-sheng,Sun, Yun.

作者其他论文 更多>>

微信小程序