Development of a stable luciferase@nanoflower-based method for the sensitive detection of pesticide residues in milk

文献类型： 外文期刊

第一作者： Li, Xiaoyu

作者： Li, Xiaoyu;Huang, Fengchun;Zhang, Juan;Chen, Jiaci;Pang, Xiangyi;Yang, Longrui;Sun, Xiaoyun;Fan, Yuhang;Chen, Ailiang;Li, Xiaoyu;Yang, Qingli

作者机构：

关键词： Luciferase; Nanoflowers; CaCl2; Bioluminescence; Pesticide residue detection

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 483 卷

页码：

收录情况： SCI

摘要： Acetylcholinesterase inhibition-luciferase bioluminescence system enables rapid detection of organophosphate and carbamate pesticide residues. However, firefly luciferase (FLuc) suffers from poor stability and short luminescence duration, thereby introducing uncertainties in detection. To address this problem, nanoflowerimmobilized FLuc (FLuc@NFs) was prepared using a coprecipitation method, which markedly enhanced stability and activity. FLuc@NFs exhibited intact nanoflower structures and allowed storage at 25 degrees C and at 4 degrees C for 2 weeks, overcoming the limitation of free enzyme storage at-20 degrees C. In the bioluminescence reaction, FLuc@NFs at 1 mg/mL achieved 62.14 % higher luminescence than free FLuc, with stable signals extending from 10 s to 30 min, enabling high-throughput detection. The system achieved detection limits of 5 and 10 ng/mL for chlorpyrifos and carbaryl, respectively, demonstrating improved reliability and sensitivity in pesticide detection.

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