Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows
文献类型: 外文期刊
第一作者: Tian, He
作者: Tian, He;Zheng, Nan;Li, Songli;Zhang, Yangdong;Wang, Jiaqi;Wang, Weiyu;Cheng, Jianbo
作者机构:
关键词: Metabolomics;Lipidomics;Heat stress;Lactating dairy cows
期刊名称:JOURNAL OF PROTEOMICS ( 影响因子:4.044; 五年影响因子:4.02 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Controlling heat stress (HS) is a global challenge for the dairy industry. However, simple and reliable biomarkers that aid the diagnoses of HS-induced metabolic disorders have not yet been identified. In this work, an integrated metabolomic and lipidomic approach using H-1 nuclear magnetic resonance and ultra-fast LC-MS was employed to investigate the discrimination of plasma metabolic profiles between HS-free and HS lactating dairy cows. Targeted detection using LC-MS in multiple reaction monitoring mode was used to verify the reliability of the metabolites as biomarker candidates. Overall, 41 metabolites were identified as candidates for lactating dairy cows exposed to HS, among which 13 metabolites, including trimethylamine, glucose, lactate, betaine, creatine, pyruvate, acetoacetate, acetone, beta-hydroxybutyrate, C16 sphinganine, lysophosphatidylcholine (18:0), phosphatidylcholine (16:0/14:0), and arachidonic acid, had high sensitivity and specificity in diagnosing HS status, and are likely to be the potential biomarkers of HS dairy cows. All of these potentially diagnostic biomarkers were involved in carbohydrate, amino acid, lipid, or gut microbiome-derived metabolism, indicating that HS affected the metabolic pathways in lactating dairy cows. Further research is warranted to evaluate these biomarkers in practical applications and to elucidate the physiological mechanisms of HS-induced metabolic disorders.
分类号: Q51
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