Development of a novel biosensor based on F0F1-ATPase for the detection of 2-dodecylcyclobutanone in irradiated beef

文献类型： 外文期刊

第一作者： Zhao, Yueliang

作者： Zhao, Yueliang;Ha, Yiming;Wang, Feng;Yue, Jiachang

作者机构：

关键词： F0F1-ATPase;Immuno-rotary biosensor;2-Dodecylcyclobutanone;Irradiated beef;GC-MS

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel biosensor regulated by the rotator of F0F1-ATPase was developed to analyze 2-dodecylcyclobutanone (2-DCB) to detect gamma-ray irradiated beef rapidly. The biosensor was assembled by conjugating 2-DCB monoclonal antibodies with the "rotator" epsilon-subunit of F0F1-ATPase within chromatophores through an E-subunit monoclonal antibody-biotin-avidin-biotin linker. The limit of detection (LOD) of 2-DCB was approximately 10(-8) mu g/mL. The recovery ratio of 2-DCB from ground beef patties ranged from 75.1% to 116.4%. The intra-assay and inter-assay coefficients of variation were both <15.0%. The proposed method was validated by gas chromatography-mass spectrometry with high correlation. The biosensor was used to detect 2-DCB in ground beef patties with different fat contents (10%, 20%, and 30%) irradiated at 0.5, 1.0, 3.0, 5.0, and 7.0 kGy. The 2-DCB concentration linearly increased with the radiation dose in all the beef samples. 2-DCB concentration increased with fat levels in the three samples. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： TS2`TS201.2

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