Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation

文献类型: 外文期刊

第一作者: Han, Xiangan

作者: Han, Xiangan;Liu, Lei;Fan, Guobo;Zhang, Yuxi;Xu, Da;Zuo, Jiakun;Wang, Shaohui;Wang, Xiaolan;Tian, Mingxing;Ding, Chan;Yu, Shengqing

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关键词: Riemerella anatipestifer;Pfs;Autoinducer-2;Biofilm formation

期刊名称:RESEARCH IN MICROBIOLOGY ( 影响因子:3.992; 五年影响因子:4.061 )

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收录情况: SCI

摘要: Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis of AI-2. In this study, we investigated the AI-2 activity of RA using an AI-2 bioassay, which showed that RA does not produce AI-2. Bioinformatic analysis indicated that the RA genome has a pfs, but not a luxS, homologue. To investigate the function of RA pfs, an avian pathogenic Escherichia colt (APEC) pfs mutant was constructed, which was subsequently transformed with a recombinant plasmid carrying RA pfs. An AI-2 bioassay demonstrated that RA pfs restored AI-2 production to the APEC pfs mutant, suggesting that RA pfs functions in AI-2 synthesis. Furthermore, we found that RA utilizes exogenous AI-2 to regulate biofilm formation. RA biofilm formation decreased significantly upon addition of exogenous AI-2. Real-time quantitative PCR results showed that expression of 13 genes related to RA biofilm formation decreased significantly when exogenous AI-2 was added to the RA culture media. These findings will benefit future studies on AI-2 regulation in RA. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

分类号: R37

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