Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation

文献类型： 外文期刊

第一作者： Han, Xiangan

作者： Han, Xiangan;Liu, Lei;Fan, Guobo;Zhang, Yuxi;Xu, Da;Zuo, Jiakun;Wang, Shaohui;Wang, Xiaolan;Tian, Mingxing;Ding, Chan;Yu, Shengqing

作者机构：

关键词： Riemerella anatipestifer;Pfs;Autoinducer-2;Biofilm formation

期刊名称：RESEARCH IN MICROBIOLOGY （ 影响因子：3.992； 五年影响因子：4.061 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis of AI-2. In this study, we investigated the AI-2 activity of RA using an AI-2 bioassay, which showed that RA does not produce AI-2. Bioinformatic analysis indicated that the RA genome has a pfs, but not a luxS, homologue. To investigate the function of RA pfs, an avian pathogenic Escherichia colt (APEC) pfs mutant was constructed, which was subsequently transformed with a recombinant plasmid carrying RA pfs. An AI-2 bioassay demonstrated that RA pfs restored AI-2 production to the APEC pfs mutant, suggesting that RA pfs functions in AI-2 synthesis. Furthermore, we found that RA utilizes exogenous AI-2 to regulate biofilm formation. RA biofilm formation decreased significantly upon addition of exogenous AI-2. Real-time quantitative PCR results showed that expression of 13 genes related to RA biofilm formation decreased significantly when exogenous AI-2 was added to the RA culture media. These findings will benefit future studies on AI-2 regulation in RA. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

分类号： R37

  • 相关文献

[1]Type IV pilus biogenesis genes and their roles in biofilm formation in the biological control agent Lysobacter enzymogenes OH11. Xia, Jing,Chen, Jiaojiao,Chen, Yuan,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan. 2018

[2]Assessment of antibiotic susceptibilities, genotypic characteristics and biofilm-forming abilities of Staphylococcus aureus and Salmonella Typhimurium. Xu, Hua,Ahn, Juhee,Xu, Hua,He, Xinlong,Ahn, Juhee. 2011

[3]Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli. Guan, Wei,Yang, Yuwen,Yan, Wanrong,Sun, Baixin,Zhao, Tingchang,Wang, Tielin,Huang, Qi.

[4]Characterization of avian pathogenic Escherichia coli isolated in eastern China. Dou, Xinhong,Gong, Jiansen,Xu, Ming,Shen, Haiyu,Zhang, Di,Liu, Jiasheng,Zou, Jianmin,Dou, Xinhong,Gong, Jiansen,Shen, Haiyu,Zou, Jianmin,Han, Xiangan,Zhuang, Linlin. 2016

[5]Comparative Proteomics Analysis of Bacillus amyloliquefaciens SQR9 Revealed the Key Proteins Involved in in Situ Root Colonization. Qiu, Meihua,Xu, Zhihui,Li, Xingxing,Li, Qing,Zhang, Nan,Shen, Qirong,Zhang, Ruifu,Qiu, Meihua,Xu, Zhihui,Li, Xingxing,Li, Qing,Zhang, Nan,Shen, Qirong,Zhang, Ruifu,Zhang, Ruifu.

[6]The Lon protease homologue LonA, not LonC, contributes to the stress tolerance and biofilm formation of Actinobacillus pleuropneumoniae. Xie, Fang,Li, Gang,Zhang, Yanhe,Zhou, Long,Liu, Shuanghong,Liu, Siguo,Wang, Chunlai.

[7]Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FIeQ and its role in biofilm formation and root colonization. Ma Yao,Ma Yao,Zhang Qiu-lei,Yang Zhi-min,Li Yun,Yan Yong-liang,Ping Shu-zhen,Zhang Li-wen,Lin Min,Lu Wei,Yang Zhi-min.

[8]A novel rhizobacterium Bk7 for biological control of brown sheath rot of rice caused by Pseudomonas fuscovaginae and its mode of action. Kakar, Kaleem Ullah,Almoneafy, Abdlwareth A.,Elshakh, Ahmed,Li, Bin,Xie, Guan-Lin,Kakar, Kaleem Ullah,Almoneafy, Abdlwareth A.,Elshakh, Ahmed,Li, Bin,Xie, Guan-Lin,Duan, Y. -P.,Nawaz, Zarqa,Sun, Guochang,Hassan, M. Auwal.

[9]Bacillomycin L and surfactin contribute synergistically to the phenotypic features of Bacillus subtilis 916 and the biocontrol of rice sheath blight induced by Rhizoctonia solani. Luo, Chuping,Zhou, Huafei,Zou, Jincheng,Wang, Xiaoyu,Zhang, Rongsheng,Xiang, Yaping,Chen, Zhiyi,Luo, Chuping,Zhou, Huafei,Xiang, Yaping,Chen, Zhiyi.

[10]Isolation of transposon mutants and characterization of genes involved in biofilm formation by Pseudomonas fluorescens TC222. Zhang, Jie,Song, Fuping,Fan, Liqiang,Huang, Dafang. 2007

[11]Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation. Wang, Xiaoxu,Li, Shiyu,Lu, Xiaoran,Li, Zheng,Bu, Zhaoyang,Lang, Xulong,Wang, Xinlong,Wang, Xiaoxu,Hu, Pan,Chen, Haiyan.

[12]Novel insights into the regulatory roles of gene hshB in Xanthomonas oryzae pv. oryzicola. Song, Zhiwei,Zhao, Yancun,Liu, Fengquan,Qian, Guoliang,Odhiambo, Benard Omondi,Qian, Guoliang,Odhiambo, Benard Omondi.

[13]Identification of genes involved in Mycoplasma gallisepticum biofilm formation using mini-Tn4001-SGM transposon mutagenesis. Wang, Yang,Zhang, Fanqing,Qiu, Xusheng,Tan, Lei,Yu, Shengqing,Ding, Chan,Wang, Yang,Cheng, Xiangchao,Yi, Li,Ding, Chan.

[14]Immunoproteomics analysis of whole cell bacterial proteins of Riemerella anatipestifer. Hu, Qinghai,Ding, Chan,Tu, Jing,Wang, Xiaolan,Han, Xiangan,Duan, Yunbing,Yu, Shengqing,Yu, Shengqing.

[15]Identification and immunological characteristics of chaperonin GroEL in Riemerella anatipestifer. Han, Xiangan,Hu, Qinghai,Chen, Wenjing,Ding, Chan,He, Liang,Wang, Xiaolan,Yu, Shengqing,Ding, Siyu,Ding, Jiabo,Yu, Shengqing.

[16]&ITRiemerella anatipestifer&IT &ITM949&IT_&IT0459&IT gene is responsible for the bacterial resistance to tigecycline. Li, Tao,Shan, Min,He, Jing,Wang, Xiaolan,Wang, Shaohui,Tian, Mingxing,Qi, Jingjing,Shi, Yonghong,Ding, Chan,Yu, Shengqing,Shan, Min,Luo, Tingrong. 2017

[17]Evaluation of a quantitative real-time PCR for rapid detection of Riemerella Anatipestifer infection in birds. Zhang, Qingshan,Li, Chenxi,Bai, Xiaofei,Liu, Ming,Liu, Siguo,Zhang, Yun,Wan, Chunhe. 2017

[18]The M949_1556 gene plays a role on the bacterial antigenicity and pathogenicity of Riemerella anatipestifer. Zou, Jiechi,Wang, Xiaolan,Tian, Mingxing,Cao, Shoulin,Hou, Wanwan,Wang, Shaohui,Han, Xiangan,Ding, Chan,Yu, Shengqing.

[19]Genome-wide mining of potential virulence-associated genes in Riemerella anatipestifer using random transposon mutagenesis. Ni, Xintao,Jiang, Pan,Xing, Linlin,Ou, Changcan,Yu, Hui,Qi, Jingjing,Sun, Bingqing,Cui, Junsheng,Hu, Qinghai,Wang, Guijun.

[20]Levamisole enhances immunity in ducklings vaccinated against Riemerella anatipestifer. Zhang, Yuewei,Zeng, Xiangfan,Wu, Wenxue,Chen, Huiling,Wang, Peng,Li, Jinxiang.

作者其他论文 更多>>

微信小程序