A peculiar segmented flow microfluidics for isoquercitrin biosynthesis based on coupling of reaction and separation

文献类型： 外文期刊

第一作者： Gong, An

作者： Gong, An;Gu, Shuang-Shuang;Wang, Jun;Sheng, Sheng;Wu, Fu-An;Gong, An;Gu, Shuang-Shuang;Wang, Jun;Sheng, Sheng;Wu, Fu-An;Wang, Jun;Sheng, Sheng;Wu, Fu-An

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关键词： Biphasic catalysis;Enzymatic synthesis;Isoquercitrin;Micro-channel reactor;Segmented flow

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

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收录情况： SCI

摘要： A segmented flow containing a buffer-ionic liquid/solvent in a micro-channel reactor was applied to synthesize isoquercitrin by the hesperidinase-catalyzed selective hydrolysis of rutin, based on a novel system of reaction coupling with separation. Within the developed microchannel reactor with one T-shaped inlet and outlet, the maximum isoquercitrin yield (101.7 +/- 2.6%) was achieved in 20 min at 30 degrees C and 4 mu L/min. Compared with a continuous-flow reactor, reaction rate was increased 4-fold due to a glycine-sodium hydroxide:[Bmim][BF4]/glycerol triacetate (1: 1, v/v) system that formed a slug flow in microchannel and significantly increased mass transfer rates. The mass transfer coefficient significantly increased and exhibited a linear relationship with the flow rate. Hesperidinase could be efficiently reused at least 5 times, without losing any activity. The bonding mechanism and secondary structure of hesperidinase indicated that hesperidinase had a greater affinity to rutin at a production rate of 4 mu L/min in this segmented flow microreactor. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： Q

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