Identification of microRNAs and their mRNA targets during soybean nodule development: functional analysis of the role of miR393j-3p in soybean nodulation

文献类型： 外文期刊

第一作者： Yan, Zhe

作者： Yan, Zhe;Hossain, Md Shakhawat;Valdes-Lopez, Oswaldo;Wang, Jun;Libault, Marc;Stacey, Gary;Yan, Zhe;Hossain, Md Shakhawat;Valdes-Lopez, Oswaldo;Wang, Jun;Libault, Marc;Stacey, Gary;Arikit, Siwaret;Zhai, Jixian;Meyers, Blake C.;Arikit, Siwaret;Zhai, Jixian;Meyers, Blake C.;Wang, Jun;Qiu, Lijuan;Ji, Tieming

作者机构：

关键词： ENOD93;microRNA (miRNA);miR393j-3p;root hair;soybean (Glycine max);symbiosis

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Plant microRNAs (miRNAs) play important regulatory roles in a number of developmental processes. The present work investigated the roles of miRNAs during nodule development in the crop legume soybean (Glycine max). Fifteen soybean small RNA libraries were sequenced from different stages of nodule development, including young nodules, mature nodules and senescent nodules. In order to identify the regulatory targets of the miRNAs, five parallel analysis of RNA ends (PARE) libraries were also sequenced from the same stages of nodule development. Sequencing identified 284 miRNAs, including 178 novel soybean miRNAs. Analysis of miRNA abundance identified 139 miRNAs whose expression was significantly regulated during nodule development, including 12 miRNAs whose expression changed >10-fold. Analysis of the PARE libraries identified 533 miRNA targets, including three nodulation-related genes and eight nodule-specific genes. miR393j-3p was selected for detailed analysis as its expression was significantly regulated during nodule formation, and it targeted a nodulin gene, Early Nodulin 93 (ENOD93). Strong, ectopic expression of miR393j-3p, as well as RNAi silencing of ENOD93 expression, significantly reduced nodule formation. The data indicate that miR393j-3p regulation of ENOD93 mRNA abundance is a key control point for soybean nodule formation.

分类号： Q94

  • 相关文献

[1]MicroRNAs Involved in the Pathogenesis of Phytophthora Root Rot of Soybean (Glycine max). Wang Jing,Zhang Li-wei,Wang Jia-lin,Chen Qing-shan,Liu Chun-yan,Hu Guo-hua,Ding Jun-jie. 2011

[2]The auxin transporter, OsAUX1, is involved in primary root and root hair elongation and in Cd stress responses in rice (Oryzasativa L.). Yu, ChenLiang,Sun, ChenDong,Wang, Suikang,Liu, Fang,Liu, Yan,Chen, YunLong,Jiang, De An,Qi, YanHua,Shen, Chenjia,Li, Chuanyou,Qian, Qian,Aryal, Bibek,Geisler, Markus.

[3]Low-P solution culture can be used for screening root growth vigor in soil for high nutrient uptake of spring wheat varieties. Wang, Yaosheng,Wang, Yaosheng,Jensen, Lars Stoumann,Magid, Jakob. 2018

[4]RSL genes are sufficient for rhizoid system development in early diverging land plants. Jang, Geupil,Pires, Nuno D.,Dolan, Liam,Jang, Geupil,Yi, Keke,Pires, Nuno D.,Menand, Benoit,Dolan, Liam,Yi, Keke,Menand, Benoit. 2011

[5]Identification and functional characterization of soybean root hair microRNAs expressed in response to Bradyrhizobium japonicum infection. Yan, Zhe,Hossain, Md Shakhawat,Valdes Lopez, Oswaldo,Hoang, Nhung T.,Wang, Jun,Libault, Marc,Brechenmacher, Laurent,Findley, Seth,Stacey, Gary,Zhai, Jixian,Sherrier, D. Janine,Meyers, Blake C.,Zhai, Jixian,Sherrier, D. Janine,Meyers, Blake C.,Wang, Jun,Qiu, Lijuan,Joshi, Trupti,Xu, Dong,Joshi, Trupti,Xu, Dong,Ji, Tieming,Yan, Zhe,Valdes Lopez, Oswaldo,Libault, Marc.

[6]Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis. Zhang, Haiwen,Zhao, Fu-Geng,Wang, Yuan,Zhang, Haiwen,Yu, Yuexuan,Song, Jiali,Li, Legong,Zhang, Haiwen,Tang, Ren-Jie,Luan, Sheng,Zhang, Haiwen.

[7]Light effect on the tissue contents and distribution of isoflavones in the developing seedling of soybean. Sun, JM,Ding, AL. 1998

[8]Association mapping of loci controlling genetic and environmental interaction of soybean flowering time under various photo-thermal conditions. Mao, Tingting,Li, Wenbin,Han, Tianfu,Mao, Tingting,Li, Jinyu,Wu, Tingting,Wu, Cunxiang,Sun, Shi,Jiang, Bingjun,Hou, Wensheng,Han, Tianfu,Wen, Zixiang,Wang, Dechun,Song, Qijian. 2017

[9]Mapping powdery mildew resistance gene in V97-3000 soybean. Wang, Yueqiang,Shi, Ainong,Zhang, Bo,Chen, Pengyin.

[10]Expression of a novel bi-directional Brassica napus promoter in soybean. Chennareddy, Siva,Cicak, Toby,Clark, Lauren,Russell, Sean,Skokut, Michiyo,Beringer, Jeffrey,Jia, Yi,Gupta, Manju,Yang, Xiaozeng.

[11]Agronomic traits of soybean cultivars released in different decades after grafting record-yield cultivar as rootstock. Li, Shengyou,Teng, Fei,Rao, Demin,Yao, Xingdong,Zhang, Huijun,Wang, Haiying,Xie, Futi,Song, Shuhong,St Martin, Steven K..

[12]Isolation and characterization of a pathogenesis-related protein 10 gene (GmPR10) with induced expression in soybean (Glycine max) during infection with Phytophthora sojae. Xu, Pengfei,Jiang, Liangyu,Li, Wenbin,Fan, Sujie,Zhang, Shuzhen,Wu, Junjiang,Wu, Junjiang.

[13]Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Xu, Min-Jun,Zhou, Dong-Hui,Huang, Si-Yang,Fan, Yi-Fan,Zhu, Xing-Quan,Nisbet, Alasdair J.,Zhu, Xing-Quan,Fan, Yi-Fan. 2013

[14]Comparative analysis of microRNAs from the lungs and trachea of dogs (Canis familiaris) infected with canine influenza virus. Su, Shuo,Zhou, Pei,Xu, Ting-Chuan,Zhang, Liang-Quan,Cao, Nan,Qi, Wen-Bao,Zhang, Gui-Hong,Li, Shou-Jun,Zhao, Fu-Rong,Zhou, Dong-Hui. 2014

[15]Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum. Shao, Chang-Chun,Tao, Jian-Ping,Shao, Chang-Chun,Xu, Min-Jun,Song, Hui-Qun,Zhu, Xing-Quan,Alasaad, Samer,Peng, Lifei. 2014

[16]Identification and characterization of microRNAs in the pancreatic fluke Eurytrema pancreaticum. Xu, Min-Jun,Huang, Si-Yang,Fu, Jing-Hua,Zhou, Dong-Hui,Zhu, Xing-Quan,Wang, Chun-Ren,Chang, Qiao-Cheng,Zheng, Xu,Zhu, Xing-Quan,Fu, Jing-Hua,Zhu, Xing-Quan. 2013

[17]Global characterization of microRNAs in Trichomonas gallinae. Xu, Min-Jun,Qiu, Shen-Ben,Fu, Jing-Hua,Zhu, Xing-Quan,Nisbet, Alasdair J.,Qiu, Shen-Ben,Fu, Jing-Hua,Shao, Chang-Chun. 2014

[18]Identification and characterization of microRNAs in Baylisascaris schroederi of the giant panda. Zhao, Guang-Hui,Xu, Min-Jun,Zhu, Xing-Quan. 2013

[19]Overexpression of microRNA gga-miR-21 in chicken fibroblasts suppresses replication of infectious bursal disease virus through inhibiting VP1 translation. Ouyang, Wei,Pan, Qun-xing,Wang, Xiao-li,Xia, Xing-xia,Bi, Zhen-wei,Wang, Yong-qiang,Wang, Xiao-mei.

[20]Identification and characterization of microRNAs by deep-sequencing in Hyalomma anatolicum anatolicum (Acari: Ixodidae) ticks. Luo, Jin,Liu, Guang-Yuan,Chen, Ze,Ren, Qiao-Yun,Yin, Hong,Luo, Jian-Xun,Wang, Hui,Yin, Hong,Wang, Hui,Wang, Hui.

作者其他论文 更多>>

微信小程序