Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale

文献类型： 外文期刊

第一作者： Sun, Ai

作者： Sun, Ai;Chen, Song-Lin;Gao, Feng-Tao;Li, Hai-Long;Liu, Xiao-Feng;Wang, Na;Sha, Zhen-Xia

作者机构：

关键词： Cynoglossus semilaevis;Flat fish;Pseudomale;Sertoli cells;GFP reporter gene transfection;RNA transfection

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A new cell line was established from half-smooth tongue sole Cynoglossus semilaevis pseudomale gonad (CSPMG). Primary culture was initiated from gonad tissues pieces, and the CSPMG cells were cultured at 24 degrees C in Dulbecco's modified Eagle medium/F12 medium (1: 1) (pH7.0), supplemented with 20 % fetal bovine serum, basic fibroblast growth factor, epidermal growth factor, insulin-like growth factor-I, 2-mercaptoethanol, penicillin and streptomycin. The cultured CSPMG cells, in fibroblast shape, proliferated to 100 % confluency 10 days later and had been subcultured to passage 109. Chromosome analyses indicated that the CSPMG cells exhibited chromosomal aneuploidy with a modal chromosome number of 42, which displayed the normal diploid karyotype of half-smooth tongue sole (2n = 42t, NF = 42). Reverse transcription polymerase chain reaction revealed CSPMG cells could express gonad somatic cell functional genes Sox9a, Wt1a and weakly germ cell marker gene Vasa, but not male specific gene Dmrt1. Transfection experiment demonstrated that CSPMG cells transfected with pEGFP-N3 plasmid and small RNA could express green and red fluorescence signals with high transfection efficiency. In conclusion, a continuous CSPMG cell line has been established successfully. The cell line might serve as a valuable tool for studies on the mechanism of sex determination, sex reversal and gonad development in flatfish.

分类号： S9

  • 相关文献

[1]PDPN gene promotes the proliferation of immature Bovine Sertoli cells in vitro. Gao, Yi,Qin, Lihong,Zhang, Guoliang,Gao, Yi,Dong, Xue,Zhao, Zijiao,Yang, Yuwei,Zhao, Zhihui.

[2]Induction of Mitogynogenetic Diploids and Identification of WW Super-female Using Sex-Specific SSR Markers in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Chen, Song-Lin,Ji, Xiang-Shan,Shao, Chang-Wei,Li, Wen-Long,Yang, Jing-Feng,Liang, Zuo,Liao, Xiao-Lin,Xu, Gen-Bo,Xu, Ying,Song, Wen-Tao,Ji, Xiang-Shan. 2012

[3]Molecular characterization and expression profiles of LPXRFa at the brain pituitary-gonad axis of half-smooth tongue sole (Cynoglossus semilaevis) during ovarian maturation. Wang, Bin,Liu, Quan,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Wang, Bin,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Liu, Quan. 2018

[4]Effects of different dietary DHA: EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis). Xu, Houguo,Cao, Lin,Wei, Yuliang,Zhang, Yuanqin,Liang, Mengqing,Liang, Mengqing. 2017

[5]Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis). Ma Qian,Zhuang Zhimeng,Feng Wenrong,Liu Shufang,Tang Qisheng. 2015

[6]Growth differences and differential expression analysis of pituitary adenylate cyclase activating polypeptide (PACAP) and growth hormone-releasing hormone (GHRH) between the sexes in half-smooth tongue sole Cynoglossus semilaevis. Ji, Xiang-Shan,Chen, Song-Lin,Xu, Tian-Jun,Yang, Jing-Feng,Tian, Yong-Sheng,Ji, Xiang-Shan,Jiang, Yun-Liang. 2011

[7]Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis. Sha, Z-X.,Luo, X-H.,Liao, X-L.,Wang, Q-L.,Chen, S-L.,Wang, S-L.,Wang, S-L.. 2011

[8]Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis). Dong Xiaoli,Chen Songlin,Shao Changwei,Dong Xiaoli,Ji Xiangshan. 2011

[9]Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis). Wang, Linna,Zhu, Ying,Xu, Wenteng,Shao, Changwei,Dong, Zhongdian,Li, Hailong,Cui, Zhongkai,Meng, Liang,Guo, Hua,Tian, Yongsheng,Chen, Songlin,Wang, Linna,Xu, Wenteng,Shao, Changwei,Tian, Yongsheng,Chen, Songlin. 2017

[10]Sensory bases and the mode of feeding behavior of tongue sole Cynoglossus semilaevis. Wang Xinan,Ma Aijun. 2009

[11]Effects of BPA and E2 on expression profiles of genes related to hypothalamic-pituitary-gonadal axis of half-smooth tongue sole Cynoglossus semilaevis. Li Fengling,Li Zhaoxin,Wang Qingyin,Zhai Yuxiu. 2013

[12]Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers. Chen, Song-Lin,Ji, Xiang-Shan,Jiang, Yun-Liang. 2010

[13]Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis). Liao, X.,Shao, C. W.,Tian, Y. S.,Chen, S. L.. 2007

[14]Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers. Liu Shuaishuai,Ye Naihao,Liu Yunguo,Liu Yunguo,Liu Shuaishuai,Guo Yinghui,Li Junfeng. 2011

[15]Two novel calreticulin-related molecules with microbial binding and phagocytosis enhancing capacity in the half-smooth tongue sole, Cynoglossus semilaevis. Wang, Guanghua,He, Shuwen,Zhang, Min,Jiang, Zengjie,Jiang, Zengjie. 2018

[16]Histopathological Liver and Testis Alterations in Male Half Smooth Tongue Sole (Cynoglossus semilaevis) Exposed to Endocrine Disruptors. Li, Fengling,Yao, Lin,Sun, Weihong,Jiang, Yanhua,Li, Zhaoxin,Zhai, Yuxiu. 2017

[17]A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome. Zhang, Junjie,Shao, Changwei,Zhang, Liyan,Liu, Kun,Gao, Fengtao,Dong, Zhongdian,Chen, Songlin,Zhang, Junjie,Zhang, Junjie,Xu, Peng. 2014

[18]Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis. Liu Yunguo,Liu Yunguo,Li Junfeng,Ye Naihao. 2011

[19]Growth and survival of tongue sole (Cynoglossus semilaevis Gunther, 1873) larvae fed a compound diet with different protein sources. Chang, Q,Liang, MQ,Wang, JL,Chen, SQ,Zhang, XM,Liu, XD. 2006

[20]Antarctic krill (Euphausia superba) meal in the diets improved the reproductive performance of tongue sole (Cynoglossus semilaevis) broodstock. Xu, H. G.,Zhao, M.,Zheng, K. K.,Wei, Y. L.,Liang, M. Q.,Xu, H. G.,Zheng, K. K.,Liang, M. Q.,Zhao, M.,Yan, L.. 2017

作者其他论文 更多>>

微信小程序