A novel ribonuclease with HIV-1 reverse transcriptase inhibitory activity purified from the fungus Ramaria formosa

文献类型： 外文期刊

第一作者： Wang, Hexiang

作者： Wang, Hexiang;Zhang, Rui;Wang, Hexiang;Tian, Guoting;Zhao, Yongchang;Zhao, Liyan;Gong, Zhiyuan;Ng, Tzi Bun

作者机构：

关键词： Ribonuclease;HIV-1 reverse transcriptase;Ramaria formosa

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A purification protocol that encompassed anion exchange chromatography (EC) on DEAE-cellulose, cation EC on CM-cellulose, anion EC on Q-Sepharose, and fast protein liquid chromatography-gel filtration of Superdex 75 was used to isolate a ribonuclease from dried fruiting bodies of Ramaria formosa. The ribonuclease was unadsorbed on CM-cellulose but adsorbed on both DEAE-cellulose and Q-Sepharose. It displayed a molecular mass of 29-kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ranking of its ribonucleolytic activity toward polyhomoribonucleotides was poly(U)>poly(C)>poly(G)>poly(A). It exhibited a pH optimum of pH 5 and a temperature optimum at 60 degrees C. The ribonuclease inhibited HIV-1 reverse transcriptase with an IC50 of 3 mu M.

分类号： R37

  • 相关文献

[1]Purification and characterization of a protein with antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities from small brown-eyed cowpea seeds. Tian, Guo-Ting,Zhu, Meng Juan,Wu, Ying Ying,Liu, Qin,Wang, He Xiang,Zhu, Meng Juan,Wu, Ying Ying,Liu, Qin,Wang, He Xiang,Ng, Tzi Bun. 2013

[2]A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea. Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Zhang, R. Y.,Ng, T. B.. 2011

[3]Overexpression of an S-like ribonuclease gene, OsRNS4, confers enhanced tolerance to high salinity and hyposensitivity to phytochrome-mediated light signals in rice. Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Li, Yaping,Liu, Qianqian,Xie, Xianzhi,Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Xie, Xianzhi. 2014

[4]First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha. Ma, Duanzheng,Wang, Hexiang,Sekete, Malota,Ma, Duanzheng,Wang, Hexiang,Wang, Bo,Ng, Tzibun. 2012

[5]Invasive ornamental plants: Problems, challenges, and molecular tools to neutralize their invasiveness. Li, Y,Cheng, ZM,Smith, WA,Ellis, DR,Chen, YQ,Zheng, XL,Pei, Y,Luo, KM,Zhao, DG,Yao, QH,Duan, H,Li, Q.

[6]Purification and characterization of a ribonuclease with antiproliferative activity from the mystical wild mushroom Tuber indicum. Feng, ShanShan,Wang, HeXiang,Xiao, Chen,Feng, ShanShan,Wang, HeXiang,Gong, ZhiYuan,Ng, TziBun.

[7]Nickel affects xylem Sap RNase a and converts RNase A to a urease. Bai, Cheng,Liu, Liping,Bai, Cheng,Wood, Bruce W.. 2013

[8]A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune. Zhang, Guo-Qing,Wang, He-Xiang,Zhang, Guo-Qing,Wang, He-Xiang,Zhao, Yong-Chang,Zhang, Guo-Qing,Ng, Tzi-Bun.

[9]An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica. Zhao Shuang,Zhang, Guo Qing,Wang, He Xiang,Zhao Shuang,Zhang, Guo Qing,Wang, He Xiang,Zhao Shuang,Zhao, Yong Chang,Li, Shu Hong,Ng, Tzi Bun.

作者其他论文 更多>>

微信小程序