Sample-pretreatment-free based high sensitive determination of aflatoxin M-1 in raw milk using a time-resolved fluorescent competitive immunochromatographic assay

文献类型： 外文期刊

第一作者： Tang, Xiaoqian

作者： Tang, Xiaoqian;Zhang, Zhaowei;Li, Peiwu;Zhang, Qi;Jiang, Jun;Wang, Du;Lei, Jiawen;Tang, Xiaoqian;Zhang, Zhaowei;Li, Peiwu;Jiang, Jun;Lei, Jiawen;Tang, Xiaoqian;Zhang, Zhaowei;Li, Peiwu;Zhang, Qi;Jiang, Jun;Lei, Jiawen;Tang, Xiaoqian;Li, Peiwu;Jiang, Jun;Lei, Jiawen;Tang, Xiaoqian;Li, Peiwu;Jiang, Jun;Wang, Du;Lei, Jiawen

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期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）

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收录情况： SCI

摘要： A highly-sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) was developed to detect aflatoxin M-1 (AFM(1)) in raw milk samples within 6 minutes without any sample pretreatments. This method could meet the requirement for rapid and sensitive milk monitoring in dairy farms and milk industries. Based on a competitive format and the home-made monoclonal antibody 2C9 against AFM(1), this assay enhanced the sensitivity from 0.3 ng mL(-1) (by using nanogold-strip method previously reported) to 0.03 ng mL(-1) (by using this TRFICA method). The improved sensitivity could probably result from the increases in both the higher affinity of monoclonal antibody 2C9 against AFM(1) and detection signals of immunoassay probes (with each europium microbead coupled with numbers of 2C9 antibodies). The TRFICA method showed a considerable dynamic range of 0.1-2.0 ng mL(-1) and spiked recoveries of 80-110% for AFM(1) quantification in raw milk. The results via TRFICA method was found to be high consistency (R-2 = 0.988) with results via standard high-performance liquid chromatography (HPLC) method, when detecting AFM(1) in 17 blind milk samples.

分类号： O6

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