Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome
文献类型: 外文期刊
第一作者: Lu, Jing
作者: Lu, Jing;van Hooijdonk, Toon;Hettinga, Kasper;Lu, Jing;Boeren, Sjef;Vervoort, Jacques;Lu, Jing
作者机构:
关键词: proteomics;metabolomics;DGAT1 polymorphism;milk synthesis
期刊名称:JOURNAL OF DAIRY SCIENCE ( 影响因子:4.034; 五年影响因子:4.354 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes the synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat content, and fatty acid composition). The mechanism of this influence has, however, not been elucidated. In this study, metabolomics (H-1-nuclear magnetic resonance) and proteomics (laser chromatography-tandem mass spectrometry) were applied to determine the serum and lipid metabolite composition and milk fat globule membrane proteome of milk samples from cows with the DGAT1 KK and AA genotypes. The milk samples from cows with the DGAT1 KK genotype contained more stomatin, sphingomyelin, choline, and carnitine, and less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related sugar, and orotic acid compared with milk samples from cows with the DGAT1 AA genotype. Based on these results, we propose that the differences between the DGAT1 genotypes may be related to stomatin-sphingomyelin lipid rafts as well as structural (cell membrane) differences in epithelial cells of the mammary gland. In conclusion, our study shows that, in addition to previously described changes in triglyceride composition, cows differing in DGAT1 polymorphism differ in their milk proteome and metabolome, which may help in further understanding the effect of the DGAT1 K232A polymorphism on milk production characteristics.
分类号: TS252
- 相关文献
作者其他论文 更多>>
-
Global investigation into the CqCYP76AD and CqDODA families in Chenopodium quinoa: Identification, evolutionary history, and their functional roles in betalain biosynthesis
作者:Li, Li;Li, Xiao'an;Guo, Huihui;He, Cailin;Lu, Jing;Ye, Xueling;Sun, Wenjun;Liu, Changying;Fan, Yu;Bai, Xue;Wu, Qi;Wu, Qi;Gao, Xiaoli;Liao, Wenhua
关键词:Betalain biosynthesis; CqCYP76AD; CqDODA; Evolutionary history; CqCYP76AD-CqDODA operon; Hairy root transformation; Quinoa
-
The impact of heating-induced lactosylation on the digestibility of lactotransferrin
作者:Lu, Jing;Liu, Shuangneng;Zhu, Tong;Xing, Lina;Liu, Jinqi;Wei, Miaohong;Zhang, Wenyuan;Pang, Xiaoyang;Zhang, Shuwen;Lv, Jiaping;Zheng, Xiaowei
关键词:Lactotransferrin; Heating; Lactosylation; In-vitro digestion
-
Effects of protein genetic variants on their phosphorylation levels, milk composition, milk proteome, and milk coagulation ability in Chinese Holstein bovine milk
作者:Zhang, Wenyuan;Zheng, Sifan;Zhu, Huiquan;Zhang, Yumeng;Pang, Xiaoyang;Lyu, Jiaping;Zhang, Shuwen;Zhang, Wenyuan;Zheng, Sifan;Hettinga, Kasper;Lu, Jing
关键词:Bovine milk; Genic variants; Coagulation properties; Polymorphism; Proteomics; Phosphorylation
-
Blue Light Attracts More Spodoptera frugiperda Moths and Promotes Their Flight Speed
作者:Yang, Haibo;Lu, Jing;Zhu, Pinhong;Sun, Yalan;Hu, Zhenjie;Li, Dingxu;Huang, Jianrong
关键词:Spodoptera frugiperda; sensitive wavelength; flight capability; light trap; light pollution
-
Enhancing the stability of mung bean-based milk: Insights from protein characteristics and raw material selection
作者:Dai, Ying;Liu, Jinqi;Xing, Lina;Zhu, Tong;Liu, Shuangneng;Lu, Jing;Zhou, Sumei;Li, Chunhong;Yan, Zheng;Zheng, Xiaowei;Wang, Li
关键词:Mung bean -based milk; Stability; Protein properties
-
Jasmonate-mediated polyamine oxidase 6 drives herbivore-induced polyamine catabolism in rice
作者:Zu, Hongyue;Bai, Weiwei;Kuai, Peng;Lu, Jing;Lou, Yonggen;Li, Ran;Zhang, Jing;Bai, Weiwei;Cheng, Jingli
关键词:rice (Oryza sativa); brown planthopper (Nilaparvata lugens); polyamine catabolism; polyamine oxidase 6; jasmonate; spermidine
-
Effects of endogenous DHA milk and exogenous DHA milk on oxidative stress and cognition in SAMP8 mice
作者:Wang, Xiaodan;Zhu, Huiquan;Chen, Baorong;Zhang, Yumeng;Zhang, Shuwen;Pang, Xiaoyang;Lv, Jiaping;Wang, Xiaodan;Kok, Akke;van Knegsel, Ariette;Kemp, Bas;Lu, Jing;Jiang, Shilong
关键词:DHA; Milk; Antioxidant; Cognition; Lipid-oxidative; Metabolites