An event-specific qualitative and real-time PCR detection of 98140 maize in mixed samples

文献类型： 外文期刊

第一作者： Song, Jun

作者： Song, Jun;Yin, Quan;Chang, Li-Juan;Wang, Dong;Liu, Wen-Juan;Lei, Shao-Rong;Zhang, Fu-Li;Song, Jun;Yin, Quan;Chang, Li-Juan;Wang, Dong;Liu, Wen-Juan;Lei, Shao-Rong;Niu, Bei;Liu, Yong

作者机构：

关键词： 98140 maize;Event-specific method;Genetically modified organism;Herbicide-resistant maize

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

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年卷期：

页码：

收录情况： SCI

摘要： The purpose of this study is to establish a reliable detection method specific for event 98140, a type of multiple herbicide-resistant corn. Based on the 3'-flanking sequence of event 98140, qualitative and quantitative PCR detection assays were developed. The results revealed the LOD of 0.05% of GM maize for qualitative PCR assay and 4 transgenic haploid genome copies for quantitative PCR assay used in this study. The LOQ was 20 transgenic haploid genome copies, and based on this, as low as 0.05% of 98140 genomic DNA could be accurately and quantitatively detected by means of the quantitative method. Two mixed corn samples, with known 98140 contents (2% and 0.5%), were used to verify the developed real-time PCR system, and the expected results were observed. The results indicated that the developed event-specific PCR methods could be used for the identification and quantification of maize line 98140. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： TS2

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