Proteomic analysis of conidia germination in Fusarium oxysporum f. sp cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana
文献类型: 外文期刊
第一作者: Deng, Gui-Ming
作者: Deng, Gui-Ming;Yang, Qiao-Song;He, Wei-Di;Li, Chun-Yu;Yang, Jing;Zuo, Cun-Wu;Gao, Jie;Sheng, Ou;Yi, Gan-Jun;Deng, Gui-Ming;Yang, Qiao-Song;He, Wei-Di;Li, Chun-Yu;Yang, Jing;Zuo, Cun-Wu;Gao, Jie;Sheng, Ou;Yi, Gan-Jun;Deng, Gui-Ming;Yang, Jing;Zuo, Cun-Wu;Gao, Jie;Lu, Shao-Yun;Yi, Gan-Jun;He, Wei-Di;Zhang, Sheng
作者机构:
关键词: Fusarium oxysporum f. sp cubense tropical race 4;Conidia germination;Banana;Quantitative proteomics;Ergosterol biosynthesis
期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Conidial germination is a crucial step of the soilborne fungus Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), a most important lethal disease of banana. In this study, a total of 3659 proteins were identified by isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic approach, of which 1009 were differentially expressed during conidial germination of the fungus at 0, 3, 7, and 11 h. Functional classification and bioinformatics analysis revealed that the majority of the differentially expressed proteins are involved in six metabolic pathways. Particularly, all differential proteins involved in the ergosterol biosynthesis pathway were significantly upregulated, indicating the importance of the ergosterol biosynthesis pathway to the conidial germination of Foc TR4. Quantitative RT-PCR, western blotting, and in vitro growth inhibition assay by several categories of fungicides on the Foc TR4 were used to validate the proteomics results. Four enzymes, C-24 sterol methyltransferase (ERG6), cytochrome P450 lanosterol C-14 alpha-demethylase (EGR11), hydroxymethylglutaryl-CoA synthase (ERG13), and C-4 sterol methyl oxidase (ERG25), in the ergosterol biosynthesis pathway were identified and verified, and they hold great promise as new targets for effective inhibition of Foc TR4 early growth in controlling Fusarium wilt of banana. To the best of our knowledge, this report represents the first comprehensive study on proteomics profiling of conidia germination in Foc TR4. It provides new insights into a better understanding of the developmental processes of Foc TR4 spores. More importantly, by host plant-induced gene silencing (HIGS) technology, the new targets reported in this work allow us to develop novel transgenic banana leading to high protection from Fusarium wilt and to explore more effective antifungal drugs against either individual or multiple target proteins of Foc TR4.
分类号: Q939.9`Q81
- 相关文献
作者其他论文 更多>>
-
Deciphering Trachinotus ovatus resistance to Streptococcus agalactiae:GWAS :GWAS identification of key SNPs and candidate genes
作者:Sun, Yi-Yao;Gao, Jie;Liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Zhu, Ke-Cheng;Xian, Lin;Zhang, Dian-Chang;Sun, Yi-Yao;Liu, Bao-Suo;Zhang, Nan;Guo, Hua-Yang;Zhu, Ke-Cheng;Xian, Lin;Zhang, Dian-Chang;Liu, Bao-Suo;Zhang, Nan;Zhu, Ke-Cheng;Zhang, Dian-Chang;Gao, Jie
关键词:GWAS; Trachinotus ovatus; Streptococcus agalactiae; SNP
-
Humoral and cellular immune responses following Omicron BA.2.2 breakthrough infection and Omicron BA.5 reinfection
作者:Zhao, Xin-Jing;Fang, Li-Qun;Zhao, Xin-Jing;Li, De-Yu;Zhang, Sheng;Gu, Hong-Jing;Chen, Jin-Jin;Xu, Qiang;Lv, Chen-Long;Jiang, Bao-Gui;Wang, Hui;Wang, Guo-Lin;Fang, Li-Qun;Ji, Bin;Peng, Hong-Hong;Shi, Chao;Shen, Yuan;Shang, Chao;Zhang, Cui-Ling;Li, Xiao;Qian, Cheng
关键词:
-
High-quality genome assemblies for two Australimusa bananas (Musa spp.) and insights into regulatory mechanisms of superior fiber properties
作者:Zhou, Run;Zhan, Ni;He, Weidi;Deng, Guiming;Dou, Tongxin;Hu, Chunhua;Bi, Fangcheng;Gao, Huijun;Dong, Tao;Liu, Siwen;Li, Chunyu;Yang, Qiaosong;Yi, Ganjun;Sheng, Ou;Zhou, Run;Wang, Shuo;Zhu, Xi-Tong;Xie, Wen-Zhao;Zheng, Yu-Yu;Wang, Lingqiang;Song, Jia-Ming;Chen, Ling-Ling;Dang, Jiangbo;Guo, Qigao
关键词:Australimusabananasx; T genome; Musaceae evolution; structural variations; fiber
-
Regulation of interfacial mechanics of soy protein via co-extraction with flaxseed protein for efficient fabrication of foams and emulsions
作者:Shao, Jiaqi;Yang, Jing;Huang, Fenghong;Chen, Yashu;Chen, Hongjian;Peng, Dengfeng;Deng, Qianchun;Shao, Jiaqi;Xiao, Junxia;Jin, Weiping;Geng, Fang
关键词:Plant protein; Co-extraction; Natural nanoparticle; Air-/oil -water interfaces; Emulsion/foam
-
Optimized fertilization mitigated carbon and nitrogen losses in a Solonchak
作者:Zhang, Sheng;Feng, Ying;Hao, Xingming;Zhang, Sheng;Feng, Ying;Hao, Xingming;Zhang, Sheng;Feng, Ying;Hao, Xingming;Liu, Ji;Liu, Ji;Liang, Yanning;Zhao, Xiaoning;Xue, Lihua
关键词:C losses; greenhouse gas; maize; N leaching; N losses
-
Genotypic and Phenotypic Characteristics of Lactic Acid Bacteria Associated with Forage Plants in the Native Grassland of Western Inner Mongolia and Their Application for Alfalfa Silage Fermentation
作者:Li, Wenlong;Li, Feng;Zhang, Chen;Gao, Jie;Tao, Ya;Tao, Ya;Tao, Ya
关键词:Inner Mongolia; natural forage plant; Lactiplantibacillus plantarum; Pediococcus pentosaceus; alfalfa silage
-
Biochar application reduced carbon footprint of maize production in the saline-alkali soils
作者:Zhang, Sheng;Feng, Ying;Hao, Xingming;Zhang, Sheng;Feng, Ying;Hao, Xingming;Zhang, Sheng;Feng, Ying;Hao, Xingming;Xue, Lihua;Liu, Ji;Xia, Longlong;Jia, Pingping;Zhao, Xiaoning
关键词:Greenhouse gas emissions; Carbon footprint; Biochar application; Saline-alkali soils