Optimization of Culture Medium for Sporulation and Biomass Production of a Nematophagous Fungus: Consideration of Nutritional and Environmental Conditions

文献类型： 外文期刊

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关键词： environmental factor;nutrition;Paecilomyces lilacinus;sporulation

期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）

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收录情况： SCI

摘要： This study reports the effects of various nutritional and environmental factors on sporulation and biomass of Paecilomyces lilacinus IPC-P. These factors included carbon and nitrogen sources, carbon-to-nitrogen ratios, mineral elements and vitamins together with water potentials, temperatures, dark/light cycles and pH. On the basis of these results, together with a two-step' cultivation and orthogonal method, the culture conditions for sporulation of this fungus were optimized. The spore suspension was inoculated on a basal medium (sucrose 19.00g/l, soy peptone 4.06g/l, K2HPO4 1.00g/l, KCl 0.50g/l, MgSO4 0.50g/l, FeSO4 0.01g/l, agar 13.00g/l) for 4days, before being transferred to a sporulation medium (dextrin 2.27g/l, urea 2.13g/l, CaCl2 3.00g/l, ZnSO(4)7H(2)O 0.01g/l, agar 13.00g/l) for a further 4days under the following environmental conditions: -3.9MPa/pH 7/light 24h/temperature 29 degrees C; these conditions were altered to -0.3MPa/pH 6/light 24h/temperature 23 degrees C in order to obtain better biomass yields. The data presented provide information on the nutrient and environmental requirements of this fungus, which will be essential for its commercial production.

分类号： Q945

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