A Specific and High-Throughput Fluorescence Polarization Immunoassay for Surveillance Screening of Clinafloxacin in Milk

文献类型: 外文期刊

第一作者: Chen, Jiahong

作者: Chen, Jiahong;Lv, Shuwei;Xu, Zhenlin;Yang, Jinyi;Shen, Yudong;Wang, Hong;Sun, Yuanming;Lei, Hongtao;Wang, Qiang

作者机构:

关键词: Clinafloxacin;Homogeneous;Fluorescence polarization immunoassay;Milk

期刊名称:FOOD ANALYTICAL METHODS ( 影响因子:3.366; 五年影响因子:3.07 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In this study, antibody specific to clinafloxacin (CLI) was generated for the first time, and a rapid and specific surveillance screening method, fluorescence polarization immunoassay (FPIA), was developed for the detection of CLI residue in milk. The developed FPIA showed a working range from 0.8 to 787.3 mu g/L with the IC50 of 25.6 mu g/L. The cross-reactivity to other fluoroquinolone drugs was negligible, which demonstrated high specificity. Good recoveries, ranged from 97.9 to 112.7 %, in spiked milk samples were obtained. The proposed FPIA was also validated by high performance liquid chromatography as a reference method, and the two methods yielded concordant results. The investigation indicated that the proposed FPIA is suitable for surveillance screening purposes to detect CLI residue in milk.

分类号: TS2

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