Cloning and functional analyses of pepper CaRKNR involved in Meloidogyne incognita resistance

文献类型： 外文期刊

第一作者： Mao, Zhenchuan

作者： Mao, Zhenchuan;Zhu, Pingping;Liu, Feng;Huang, Yonghong;Ling, Jian;Chen, Guohua;Yang, Yuhong;Feng, Dongxin;Xie, Bingyan

作者机构：

关键词： Capsicum annuum L.;Meloidogyne incognita;Resistance gene;Cloning;Function analysis

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Root-knot nematodes (Meloidogyne spp.) are destructive pests of crops. Pepper (Capsicum annuum L.) contains genes that control resistance to root-knot nematodes. Using suppression subtractive hybridization and RACE strategies, a nucleotide-binding site and leucine-rich repeat (NBS-LRR) family gene, CaRKNR (FJ231739), was isolated and cloned from the nematode-resistant pepper line HDA149. CaRKNR is a novel NBS-LRR gene with an open reading frame of 3600 bp that is homologous (70.45 % identity) to the gene Mi-1.2. After Meloidogyne incognita inoculation, real-time qPCR showed that the CaRKNR expression level was increased from 0.63 to 2.16 times. Using the virus-induced gene silencing system, the CaRKNR gene's expression level was reduced significantly than controls, and the average numbers of galls and egg masses in silenced seedlings were 44.39 and 42.01, respectively, while the controls were 0.13. This study revealed that CaRKNR was induced by M. incognita and its expression correlated with pepper resistance against root-knot nematodes.

分类号： S3

  • 相关文献

[1]Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility. Dai, X. Z.,Zhang, Z. Q.,Zou, X. X.,Deng, M. H.,Zhu, H. S.,Zhou, H.,Wen, J. F.,Huo, J. L.. 2012

[2]Molecular cloning of a potential Verticillium dahliae resistance gene SlVe1 with multi-site polyadenylation from Solanum licopersicoides. Zhao, LX,Liao, ZH,Sun, XF,Zuo, KJ,Zhang, L,Wang, SG,Tang, KX.

[3]Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia. Wan, Yongqing,Mao, Mingzhu,Yang, Qi,Li, Guojing,Wang, Ruigang,Wan, Dongli,Yang, Feiyun,Mandlaa. 2018

[4]Gene cloning and function analysis of ABP9 protein which specifically binds to ABRE2 motif of maize Cat1 gene. Wang, L,Zhao, J,Fan, YL. 2002

[5]Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants. Zheng, Yusheng,Wang, Zhekui,Li, Dongdong,Xu, Li,Zhou, Peng,Ye, Rongjian,Lin, Yongjun,Ye, Rongjian,Lin, Yongjun. 2010

[6]Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max) and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress. Hao, Qingnan,Shang, Weijuan,Zhang, Chanjuan,Chen, Haifeng,Chen, Limiao,Yuan, Songli,Zhou, Xinan,Hao, Qingnan,Shang, Weijuan,Zhang, Chanjuan,Chen, Haifeng,Chen, Limiao,Yuan, Songli,Chen, Shuilian,Zhang, Xiaojuan,Zhou, Xinan. 2016

[7]Transcriptome profile of liver at different physiological stages reveals potential mode for lipid metabolism in laying hens. Li, Hong,Wang, Taian,Xu, Chunlin,Wang, Dandan,Ren, Junxiao,Li, Yanmin,Tian, Yadong,Wang, Yanbin,Jiao, Yuping,Kang, Xiangtao,Liu, Xiaojun,Tian, Yadong,Wang, Yanbin,Kang, Xiangtao,Liu, Xiaojun,Kang, Xiangtao,Liu, Xiaojun,Jiao, Yuping. 2015

[8]Overexpression of the autophagy-related gene SiATG8a from foxtail millet (Setaria italica L.) confers tolerance to both nitrogen starvation and drought stress in Arabidopsis. Li, Wei-wei,Liu, Jia-ming,Guo, Chang-Hong,Chen, Ming,Xu, Zhao-shi,Li, Lian-cheng,Zhou, Yong-Bin,Ma, You-Zhi,Zhong, Li.

[9]Progress of reverse genetics technique in influenza virus. Liu Da-Fei,Liu Chun-Guo,Liu Ming,Liu Da-Cheng. 2008

[10]Effect of continuous negative pressure water supply on the growth, development and physiological mechanism of Capsicum annuum L.. Li Di,Shao Hong-ying,Wang Peng,Long Huai-yu,Zhang Shu-xiang,Wu Xue-ping. 2017

[11]Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight. Xie, L. B.,Wang, X.,Zhou, Y.,Chen, L. X.,Gao, Y. L.,Guo, Y. H.,Peng, M.,Liu, L. X.. 2017

[12]A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.). Wang, L. H.,Gu, X. H.,Hua, M. Y.,Mao, S. L.,Zhang, Z. H.,Zhang, B. X.,Gu, X. H.,Hua, M. Y.,Yun, X. F.,Peng, D. L..

[13]RELATIONSHIP OF ASCORBIC ACID METABOLISM WITH THE CYTOPLASMIC MALE STERILITY IN PEPPER (CAPSICUM ANNUUM L.). Deng, Minghua,Deng, Minghua,Huo, Jinlong,Zhu, Haishan,Lv, Junheng,Dai, Xiongze,Zhang, Zhuqing,Zou, Xuexiao. 2015

[14]Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress. Guo, Meng,Liu, Jin-Hong,Lu, Jin-Ping,Zhai, Yu-Fei,Wang, Hu,Gong, Zhen-Hui,Lu, Ming-Hui,Wang, Shu-Bin. 2015

[15]Molecular characteristics and expression of cytoplasmic genes in cytoplasmic male sterility of pepper (Capsicum annuum L.). Zhang Zhuqing,Dai Xiongze,Zou Xuexiao,Ou Lijun,Ou Lijun. 2012

[16]Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L.. Cao Ya-cong,Sul Xiao-lei,Zhang Zhen-xian,Cao Ya-cong,Zhang Zheng-hai,Wang Li-hao,Zhang Bao-xi. 2016

[17]Development of pepper SSR markers from sequence databases. Huang, SW,Zhang, BX,Milbourne, D,Cardle, L,Yang, GM,Guo, JZ. 2001

[18]Cloning and characterization of the CarbcL gene related to chlorophyll in pepper (Capsicum annuum L.) under fruit shade stress. Wang, Shu-Bin,Pan, Bao-Gui,Diao, Wei-Ping,Tian, Shi-Lin,Shah, Syed N. M.,Gong, Zhen-Hui,Tian, Shi-Lin,Shah, Syed N. M.. 2015

[19]Characterization and expression profile of CaNAC2 pepper gene. Guo, Wei-Li,Chen, Ru-Gang,Yin, Yan-Xu,Gong, Zhen-Hui,Guo, Wei-Li,Chen, Bi-Hua,Du, Xiao-Hua,Zhang, Yu-Yuan,Wang, Shu-Bin. 2015

[20]Overexpression of the CaTIP1-1 Pepper Gene in Tobacco Enhances Resistance to Osmotic Stresses. Yin, Yan-Xu,Xiao, Huai-Juan,Zhang, Huai-Xia,Zhang, Zhen,Jing, Hua,Zhang, Ying-Li,Chen, Ru-Gang,Gong, Zhen-Hui,Wang, Shu-Bin,Zhang, Ying-Li. 2014

作者其他论文 更多>>

微信小程序