Diversifying Sunflower Germplasm by Integration and Mapping of a Novel Male Fertility Restoration Gene

文献类型： 外文期刊

第一作者： Liu, Zhao

作者： Liu, Zhao;Feng, Jiuhuan;Seiler, Gerald J.;Cai, Xiwen;Wang, Dexing;Jan, Chao-Chien

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期刊名称：GENETICS （ 影响因子：4.562； 五年影响因子：4.845 ）

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收录情况： SCI

摘要： The combination of a single cytoplasmic male-sterile (CMS) PET-1 and the corresponding fertility restoration (Rf) gene Rf(1) is used for commercial hybrid sunflower (Helianthus annuus L., 2n = 34) seed production worldwide. A new CMS line 514A was recently developed with H. tuberosus cytoplasm. However, 33 maintainers and restorers for CMS PET-1 and 20 additional tester lines failed to restore the fertility of CMS 514A. Here, we report the discovery, characterization, and molecular mapping of a novel Rf gene for CMS 514A derived from an amphiploid (Amp H. angustifolius/P 21, 2n = 68). Progeny analysis of the male-fertile (MF) plants (2n = 35) suggested that this gene, designated Rf(6), was located on a single alien chromosome. Genomic in situ hybridization (GISH) indicated that Rf(6) was on a chromosome with a small segment translocation on the long arm in the MF progenies (2n = 34). Rf(6) was mapped to linkage group (LG) 3 of the sunflower SSR map. Eight markers were identified to be linked to this gene, covering a distance of 10.8 cM. Two markers, ORS13 and ORS1114, were only 1.6 cM away from the gene. Severe segregation distortions were observed for both the fertility trait and the linked marker loci, suggesting the possibility of a low frequency of recombination or gamete selection in this region. This study discovered a new CMS/Rf gene system derived from wild species and provided significant insight into the genetic basis of this system. This will diversify the germplasm for sunflower breeding and facilitate understanding of the interaction between the cytoplasm and nuclear genes.

分类号： R394

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