Sensitivity of Corynespora cassiicola to Carbendazim and Diethofencarb

文献类型: 外文期刊

第一作者: Huang, Daye

作者: Huang, Daye;Wang, Weiping;Shi, Yanxia;Xie, Xuewen;Li, Baoju

作者机构:

关键词: Corynespora cassiicola;Carbendazim;Diethofencarb;beta-tubulin

期刊名称:JOURNAL OF PURE AND APPLIED MICROBIOLOGY ( 影响因子:0.073; )

ISSN:

年卷期:

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收录情况: SCI

摘要: During 2005 and 2011, 159 isolates of Corynespora cassiicola were collected from 10 different provinces in China, and their sensitivities to carbendazim and diethofencarb were determined. Among all isolates, 8.2% were sensitive to carbendazim (M-s), 91.8% were highly resistant to carbendazim (M-HR) and 14.5% were highly resistant to both carbendazim and diethofencarb ((MNHR)-N-HR) moderately resistant isolates to carbendazim were not found. This is the first report of C. cassiicola isolates that were highly resistant to both carbendazim and diethofencarb. As compared to M-s isolates, M-HR and (MNHR)-N-HR isolates showed similar fitness in fungal growth rate, sporulation and pathogenicity. Carbendazim resistance correlated with a single mutation in beta-tubulin gene C. cassiicola amplified with primer pair beta-tubf1-F and beta-tubf1-R causing a change of glutamic to alanine at amino acid 198. Furthermore, the substitution of glutamic acid to lysine and phenylalanine to tyrosine led the resistance to both fungicides at amino acid 198 and 200 repecetively.

分类号: Q93

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