Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle

文献类型： 外文期刊

第一作者： Lu, Feng-Gong

作者： Lu, Feng-Gong;Fu, Kai-Yun;Li, Guo-Qing;Guo, Wen-Chao

作者机构：

关键词： Leptinotarsa decemlineata;Juvenile hormone epoxide hydrolase;RNA interference;Juvenile hormone titer;Performance

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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年卷期：

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收录情况： SCI

摘要： In insect, juvenile hormone OH) titers are tightly regulated in different development stages through synthesis and degradation pathways. During JH degradation, JH epoxide hydrolase (JHEH) converts JH to JH diol, and hydrolyses JH acid to JH acid diol. In this study, two full length LdJHEH cDNAs were cloned from Leptinotarsa decemlineata, and were provisionally designated LdJHEH1 and LdJHEH2. Both mRNAs were detectable in the thoracic muscles, brain-corpora cardiaca-corpora allata complex, foregut, midgut, hindgut, ventral ganglia, Malpighian tubules, fat bodies, epidermis, and hemocytes of the day 2 fourth-instar larvae, and in female ovaries as well as male reproductive organs of the adults. Moreover, both LdJHEH1 and LdJHEH2 were expressed throughout all larval life, with the highest peaks occurring 32 h after ecdysis of the final (fourth) instar larvae. Four double-stranded RNAs (dsRNAs) (d5JHEH1-1, dsJHEH1-2, ds1HEH2-1, dsJHEH2-2) respectively targeting LdJHEH1 and LdJHEH2 were constructed and bacterially expressed. Ingestion of dsJHEH1-1, dsJHEH1-2, dsJHEH2-1, dsJHEH2-2, and a mixture of dsJHEH1-1 + d5JHEH2-1 successfully knocked down corresponding target gene function, and significantly increased JH titer and upregulated Kriippel homolog 1 (LdKr-h1) mRNA level. Knockdown of either LdJHEH1 or LdJHEH2, or both genes slightly reduced larval weight and delayed larval development, and significantly impaired adult emergence. Therefore, it is suggested that knockdown LdJHEH1 and LdJHEH2 affected JH degradation in the Colorado potato beetle. (C) 2015 Elsevier B.V. Al! rights reserved.

分类号： R394

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