A suicidal DNA vaccine expressing the fusion protein of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in mice

文献类型： 外文期刊

第一作者： Wang, Yong

作者： Wang, Yong;Yue, Xiaolin;Pan, Ling;Wang, Guijun;Guo, Hao;Jin, Hongyan;Li, Gang;Liu, Guangqing;Li, Yongdong;Wang, Yong

作者机构：

关键词： Fusion protein;Immune response;Peste des petits ruminants virus;Suicidal DNA vaccine

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Peste des petits ruminants (PPR), a highly contagious disease induced by PPR virus (PPRV), affects sheep and goats. PPRV fusion (F) protein is important for the induction of immune responses against PPRV. We constructed a Semliki Forest virus (SFV) replicon-vectored DNA vaccine ("suicidal DNA vaccine") and evaluated its immunogenicity in BALB/c mice. The F gene of PPRV was cloned and inserted into the SFV replicon-based vector pSCA1. The antigenicity of the resultant plasmid pSCA1/F was identified by indirect immunofluorescence and western blotting. BALB/c mice were then intramuscularly injected with pSCA1/F three times at 14-d intervals. Specific antibodies and virus-neutralizing antibodies against PPRV were quantified by indirect ELISA and microneutralization tests, respectively. Cell-mediated immune responses were examined by cytokine and lymphocyte proliferation assays. The pSCA1/F expressed F protein in vitro and induced specific and neutralizing antibody production, and lymphocyte proliferation in mice. Mice vaccinated with pSCA1/F had increased IL-2 and IL-10 levels after 24-h post first immunization. IFN-gamma and TNF-alpha levels increased from that time point and gradually decreased thereafter. Thus, the Semliki Forest virus replicon-vectored DNA vaccine expressing the F protein of PPRV induced both humoral and cell-mediated immune responses in mice. This could be considered as a novel strategy for vaccine development against PPR. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R37

  • 相关文献

[1]Immune responses in mice vaccinated with a suicidal DNA vaccine expressing the hemagglutinin glycoprotein from the peste des petits ruminants virus. Wang, Yong,Shi, Lijun,Li, Wenchao,Jin, Hongyan,Li, Gang,Xu, Binrui,Liu, Guangqing,Li, Chuanfeng,Chen, Zongyan,Wang, Yong. 2013

[2]Recombinant adenovirus expressing F and H fusion proteins of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in goats. Wang, Yong,Xu, Binrui,Wang, Yong,Shi, Lijun,Li, Wenchao,Huang, Huaxin,Tao, Chunai,Cheng, Chaofei,Li, Gang,Liu, Guangqing,Chen, Zongyan,Li, Chuanfeng. 2013

[3]Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein. Qi, Ting,Cui, Shang-jin.

[4]Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus. Zhu, Yingqi,Li, Chuanfeng,Bi, Zhuangli,Chen, Zongyan,Meng, Chunchun,Ding, Chan,Liu, Guangqing,Zhu, Yingqi,Bi, Zhuangli,Wang, Gui-Jun.

[5]Protective immune responses in ducklings induced by a suicidal DNA vaccine of the VP1 gene of duck hepatitis virus type 1. Fu, Yuzhi,Chen, Zongyan,Li, Chuanfeng,Liu, Guangqing,Fu, Yuzhi. 2012

[6]Protective immune responses in rabbits induced by a suicidal DNA vaccine of the VP60 gene of rabbit hemorrhagic disease virus. Cheng, Yingjie,Chen, Zongyan,Li, Chuanfeng,Meng, Chun,Liu, Guangqing,Cheng, Yingjie,Wu, Run.

[7]Diagnosis of Peste des Petits Ruminants in Wild and Domestic Animals in Xinjiang, China, 2013-2016. Li, J.,Li, L.,Wu, X.,Liu, F.,Zou, Y.,Wang, Q.,Liu, C.,Bao, J.,Wang, Z.,Wang, W.,Ma, W.,Lin, H.,Huang, J.,Zheng, X.. 2017

[8]Analysis of codon usage in peste des petits ruminant's virus. Liu, Xin-sheng,Wang, Yong-lu,Zhang, Yong-guang,Fang, Yu-zhen,Pan, Li,Lu, Jian-liang,Zhou, Peng,Zhang, Zhong-wang,Jiang, Shou-tian. 2011

[9]Enzymatic reporting of peste des petits ruminants virus genes ligating two specific probes on nanoparticles. Tao, Chunai,Li, Gang,Wang, Yong,Huang, Huaxin,Tao, Chunai,Li, Gang,Wang, Yong,Huang, Huaxin,Wang, Yong.

[10]M protein is sufficient for assembly and release of Peste des petits ruminants virus-like particles. Wang, Qiuxia,Ou, Changbo,Liu, Xingyou,Yu, Yan,Jiang, Jinqing,Ma, Jinyou,Hu, Jianhe,Wang, Qiuxia,Dou, Yongxi,Chen, Lei,Meng, Xuelian,Zhang, Zhidong,Cai, Xuepeng,Cai, Xuepeng.

[11]Development of an Indirect ELISA with Artificially Synthesized N Protein of PPR Virus. Zhu, Yu-min,Dong, Shi-juan,Yu, Rui-song,Li, Zhen,Zhu, Yu-min,Dong, Shi-juan,Yu, Rui-song,Li, Zhen,Zhang, Guo-rui,Lei, Zhi-hai,Zeng, Jiang-yong,Zhu, Se,Duoji, Ciren. 2012

[12]First report of peste des petits ruminants virus lineage II in Hydropotes inermis, China. Zhou, X. Y.,Zhu, J.,Miao, Q. -H.,Liu, G. Q.,Zhou, X. Y.,Wang, Y.,Wang, G. J.,Zhu, L. Q.,Zhan, S. H.. 2018

[13]Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus. Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Lei, Zhi-Hai,Zeng, Jiang-Yong,Zhu, Se,Duoji, Ciren,Dunzhu, Luobu. 2013

[14]Fine mapping and conservation analysis of linear B-cell epitopes of peste des petits ruminants virus hemagglutinin protein. Yu, Ruisong,Zhu, Rui,Gao, Weixiang,Dong, Shijuan,Chen, Bingqing,Yu, Li,Xie, Chunfang,Jiang, Fengying,Li, Zhen,Zhu, Rui,Gao, Weixiang,Zhang, Ming.

[15]Genome characterization and phylogenetic analysis of a lineage IV peste des petits ruminants virus in southern China. Li, Xiao-Peng,He, Dong-Sheng,Luo, Man-Lin,Chen, Rui-Ai,Zhai, Shao-Lun,Lv, Dian-Hong,Wen, Xiao-Hui,Wei, Wen-Kang,Guo, Peng-Ju.

[16]A novel fusion protein-based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis. Liu, Siguo,Guo, Sheping,Wang, Chunlai,Shao, Meili,Zhang, Xiuhua,Guo, Yang,Gong, Qiang. 2007

[17]Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens. Ji, Yanhong,Liu, Tao,Jia, Yane,Liu, Bin,Cui, Xiaole,Guo, Fengfeng,Chang, Huiyun,Zhu, Qiyun,Yu, Qingzhong.

[18]Expression and purification of half-smooth tongue sole (Cynoglossus semilaevis) CSDAZL protein. Wang, Kailin,Zhang, Hong,Hu, Qiaomu,Shao, Changwei,Chen, Songlin,Wang, Kailin.

[19]Quantitative investigation of the direct interaction between Hemagglutinin and fusion proteins of Peste des petits ruminant virus using surface Plasmon resonance. Meng, Xuelian,Deng, Ruixue,Zhu, Xueliang,Zhang, Zhidong. 2018

[20]Expression and characterization of recombinant gallinacin-9 and gallinacin-8 in Escherichia coli. Ma, De-ying,Shan, An-shan,Liu, Sheng-wang,Han, Zong-xi,Li, Yi-jin.

作者其他论文 更多>>

微信小程序