Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dur)

文献类型： 外文期刊

第一作者： Tan, Yong-An

作者： Tan, Yong-An;Xiao, Liu-Bin;Zhao, Jing;Sun, Yang;Bai, Li-Xin

作者机构：

关键词： Apolygus lucorum;Ecdysone receptor;Expression profile;RNA interference

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We cloned the cDNA of the ecdysone receptor (EcR) isoform-A from the mind bug, Apolygus lucorum (AlEcR-A). The AlEcR-A cDNA has an open reading frame of 1410 bp with a conserved sequence of approximately 20 amino acids at the carboxyl-end of its A/B-specific domain. Phylogenetic analysis showed that AlEcR-A is very similar to the EcR-A genes of other Hemiptera species. AlEcR-A mRNA was detected at all developmental stages of A. lucorum with peaks correlating to ecdysteroid pulses. AlEcR-A was also expressed in all analyzed tissues with maximum expression in the epidermis and fat body. An AlEcR-A mRNA of size 1.8 kb was detected in all tissues by northern blot analysis. We investigated the functions of AlEcR-A in A. lucorum growth and development using RNAi in vivo. Weights of fifth instar nymphs were significantly decreased in insects treated with AlEcR-A specific anti-sense RNA. Mortality from third instar nymphs to adults increased significantly along with a significant increase in instar duration. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R394

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