Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons

文献类型: 外文期刊

第一作者: Li, Liwei

作者: Li, Liwei;Zhou, Yanjun;Gao, Fei;Jiang, Yifeng;Yu, Lingxue;Zheng, Hao;Tong, Wu;Yang, Shen;Zheng, Haihong;Shan, Tongling;Liu, Fei;Xia, Tianqi;Tong, Guangzhi;Wei, Zuzhang;Zhou, Yanjun;Gao, Fei;Tong, Guangzhi

作者机构:

关键词: miR-26a;PRRSV;Type I interferon;Virus replication

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

ISSN:

年卷期:

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收录情况: SCI

摘要: MicroRNAs (miRNAs) play important roles in viral infections, especially by modulating the expression of cellular factors essential to viral replication or the host innate immune response to infection. To identify host miRNAs important to controlling porcine reproductive and respiratory syndrome virus (PRRSV) infection, we screened 15 miRNAs that were previously implicated in innate immunity or antiviral functions. Over-expression of the miR-26 family strongly inhibited PRRSV replication in vitro, as shown by virus titer assays, Western blotting, and qRT-PCR assays. MiR-26a inhibited the replication of both type 1 and type 2 PRRSV strains. Mutating the seed region of miR-26 restored viral titers. Luciferase reporters showed that miR-26a does not target the PRRSV genome directly but instead affects the expression of type I interferon and the IFN-stimulated genes MX1 and ISG15 during PRRSV infection. These results demonstrate the important role of miR-26a in modulating PRRSV infection and also support the possibility of using host miR-26a to achieve RNAi-mediated antiviral therapeutic strategies. (C) 2014 Elsevier B.V. All rights reserved.

分类号: R37

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