Transcription factor C/EBP beta and 17 beta-estradiol promote transcription of the porcine p53 gene

文献类型: 外文期刊

第一作者: Tao, Hu

作者: Tao, Hu;Zhang, Xuying;Yang, Jiahao;Zhu, Lihua;Zhou, Jiawei;Wang, Lei;Hua, Lun;Li, Fenge;Tao, Hu;Zhang, Xuying;Yang, Jiahao;Zhu, Lihua;Zhou, Jiawei;Wang, Lei;Hua, Lun;Li, Fenge;Mei, Shuqi;Peng, Xianwen;Wu, Huayu

作者机构:

关键词: Pig;p53;C/EBP beta;17 beta-Estradinl;Litter size

期刊名称:INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY ( 影响因子:5.085; 五年影响因子:4.407 )

ISSN:

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页码:

收录情况: SCI

摘要: The tumor protein 53 (p53) gene played a crucial role in maternal reproduction except its classic roles in maintaining genomic stability and preventing tumorigenesis. However, little is known concerning the regulatory elements which control the expression of p53 gene. In this study, we predicted two binding sites (-490/-477 and -405/-392) of transcription factor CCAAT/enhancer binding protein beta (C/EBP beta) within the core promoter (-985/-273) determined by promoter deletion analysis, and discovered that the second site (-405/-392) was important for p53 promoter activity by site-directed mutagenesis. Then the binding of C/EBPP to the p53 promoter was identified by electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP). Moreover, evidence from C/EBP beta overexpression and RNAi studies showed C/EBP beta regulated p53 promoter activity and endogenous p53 expression. Meanwhile, we observed p53 mRNA at the peak in 10(-6) mol/L 17 beta-estradiol treated cells for 24 h via enhancing its core promoter activity. Taken together, our study indicates that C/EBP beta and 17 beta-estradiol are the essential regulatory factors for p53 transcription. (C) 2013 Elsevier Ltd. All rights reserved.

分类号: Q5

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