Complementary transcriptomic and proteomic analyses of a chlorophyll-deficient tea plant cultivar reveal multiple metabolic pathway changes

文献类型: 外文期刊

第一作者: Wang, Lu

作者: Wang, Lu;Cao, Hongli;Yue, Chuan;Hao, Xinyuan;Yang, Yajun;Wang, Xinchao;Wang, Lu;Cao, Hongli;Yue, Chuan;Hao, Xinyuan;Yang, Yajun;Wang, Xinchao;Chen, Changsong

作者机构:

关键词: Chlorina;Chlorophyll deficiency;Proteome;ITRAQ;Microarray;Tea plant (Camellia sinensis)

期刊名称:JOURNAL OF PROTEOMICS ( 影响因子:4.044; 五年影响因子:4.02 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To uncover the mechanisms that underlie the chlorina phenotype of the tea plant, this study employs morphological, biochemical, transcriptomic, and iTRAQ-based proteomic analyses to compare the green tea cultivar LJ43 and the yellow-leaf tea cultivar ZH1. ZH1 exhibited the chlorina phenotype, with significantly decreased chlorophyll content and abnormal chloroplast development compared with LJ43. ZH1 also displayed higher theanine and free amino acid content and lower carotenoid and catechin content. Microarray and iTRAQanalyses indicated that the differentially expressed genes and proteins could be mapped to the following pathways: 'phenylpropanoid biosynthesis,' 'glutathione metabolism,' 'phenylalanine metabolism,' 'photosynthesis,' and 'flavonoid biosynthesis.' Altered gene and protein levels in these pathways may account for the increased amino acid content and reduced chlorophyll and flavonoid content of ZH1. Altogether, this study combines transcriptomic and proteomic approaches to better understand the mechanisms responsible for the chlorina phenotype. (C)2015 Elsevier B.V. All rights reserved.

分类号: Q51

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