Transformation of iaaM Gene into Anther-Derived Embryos of Loquat Mediated by Agrobacterium tumefaciens

文献类型： 外文期刊

第一作者： Wang, Y. Q.

作者： Wang, Y. Q.;Pan, C. P.;Yang, Z. W.;Deng, Q. X.;Liu, J.;Tao, L.

作者机构：

关键词： culture media;Eriobotrya japonica;genetic transformation;growth regulator;parthenocarpy gene

期刊名称：IV INTERNATIONAL SYMPOSIUM ON LOQUAT

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年卷期：

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收录情况： SCI

摘要： The parthenocarpic gene iaaM was transferred into anther-derived embryos, using Agrobacterium-mediated transformation. The results showed that 2-3-day pre-culture, 15-min infection and 3-day co-culture with 10 mg L~(-1) AS in the co-culture medium ledto an increase in transformation efficiency. Cefotaxime at 250 mg L~(-1) or Ampicillin at 750 mg L~(-1) were optimal. Sixteen transgenic lines were obtained. The iaaM gene-specific amplification results showed that 9 strains were amplified at the expected target bands, and the PCR positive rate was 56.3%. The PCR-Southern analysis indicated that 8 strains showed hybridization signal, which further proved that the iaaM gene was integrated into the genome of the embryos, and the transformation rate was about 0.5%.

分类号： S6

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